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Product Info Summary
| SKU: | A04343 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Sprouty-4 SPRY4 Antibody
SKU/Catalog Number
A04343
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-Sprouty-4 SPRY4 Antibody (Catalog # A04343). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-Sprouty-4 SPRY4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04343)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 275-299 of Human Sprouty-4 protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A04343 is reactive to SPRY4 in Human, Mouse
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
32541 MW
Background of Spry4
Sprouty-4 (also known as SPRY4) is an inhibitor of the insulin receptor and EGFR-transduced mitogen-activated protein kinase (MAPK) signaling pathway downstream of FGF and EGF receptor tyrosine kinase activation. It is positioned upstream of RAS activation and impairs the formation of active GTP-RAS. SPRY4 is widely expressed, with different isoforms. The protein consists of 322 amino acids, with a cysteine-rich region, Src homology 3 binding regions, proline-rich regions, and a PEST sequence. It is expressed predominantly in the cytoplasm. Northern results show bands across most tissues, with strongest expression in heart, brain, placenta, lung, and intestine.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04343 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:3,000 - 1:12,000
IHC: 1 µg/ml to 10 µg/ml
WB: 1:100 - 1:1,000
Validation Images & Assay Conditions
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Sprouty-4 was detected in paraffin-embedded sections of human heart tissue using rabbit anti-Sprouty-4 Antigen Affinity purified polyclonal antibody (Catalog # A04343) at 2.5 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022). Staining: Sprouty-4 as precipitated red signal with hematoxylin purple nuclear counterstain.
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Sprouty-4 was detected in paraffin-embedded sections of human liver tissue using rabbit anti-Sprouty-4 Antigen Affinity purified polyclonal antibody (Catalog # A04343) at 2.5 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022). Staining: Sprouty-4 as precipitated brown with blue nuclear counterstain.
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Western blot analysis of Sprouty-4 expression in HeLa cell lysates (lane 1), SW13 cell lysates (lane 2) and C2C12 cell lysates (lane 3). Sprouty-4 at 35KD was detected using rabbit anti-Sprouty-4 Antigen Affinity purified polyclonal antibody (Catalog # A04343) at 1:100.
Protein Target Info & Infographic
Gene/Protein Information For SPRY4 (Source: Uniprot.org, NCBI)
Gene Name
SPRY4
Full Name
Protein sprouty homolog 4
Weight
32541 MW
Superfamily
sprouty family
Alternative Names
Sprouty4, Sprouty-4, Sprouty 4, SPRY4, SPRY 4, Spry-4, Protein sprouty homolog 4 Spry4|A030006O18Rik, spro, sprouty4|sprouty RTK signaling antagonist 4|protein sprouty homolog 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SPRY4, check out the SPRY4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SPRY4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Sprouty-4 SPRY4 Antibody (A04343)
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No publications found for A04343
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-Sprouty-4 SPRY4 Antibody
Question
See attached the WB image, lot number and protocol we used for brain using anti-Sprouty-4 antibody A04343. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-05-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-05-04
Question
Is a blocking peptide available for product anti-Sprouty-4 antibody (A04343)?
N. Jones
Verified customer
Asked: 2020-02-24
Answer
We do provide the blocking peptide for product anti-Sprouty-4 antibody (A04343). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-02-24
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-Sprouty-4 antibody A04343. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-10
Question
I see that the anti-Sprouty-4 antibody A04343 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-04
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-11-04
Question
Is this A04343 anti-Sprouty-4 antibody reactive to the isotypes of SPRY4?
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
The immunogen of A04343 anti-Sprouty-4 antibody is A synthetic peptide corresponding to amino acids 306-322 of Human Sprouty-4 protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-25
Question
My question regarding product A04343, anti-Sprouty-4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-01
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04343 anti-Sprouty-4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-01
Question
Does A04343 anti-Sprouty-4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
It shows on the product datasheet, A04343 anti-Sprouty-4 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-07
Question
Would anti-Sprouty-4 antibody A04343 work for IHC with brain?
Verified Customer
Verified customer
Asked: 2018-07-13
Answer
According to the expression profile of brain, SPRY4 is highly expressed in brain. So, it is likely that anti-Sprouty-4 antibody A04343 will work for IHC with brain.
Boster Scientific Support
Answered: 2018-07-13
Question
I am looking for to test anti-Sprouty-4 antibody A04343 on human brain for research purposes, then I may be interested in using anti-Sprouty-4 antibody A04343 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-07-03
Answer
The products we sell, including anti-Sprouty-4 antibody A04343, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-07-03
Question
Will anti-Sprouty-4 antibody A04343 work on canine IHC with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2018-02-12
Answer
Our lab technicians have not validated anti-Sprouty-4 antibody A04343 on canine. You can run a BLAST between canine and the immunogen sequence of anti-Sprouty-4 antibody A04343 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine cervix carcinoma in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-02-12
Question
Is there a BSA free version of anti-Sprouty-4 antibody A04343 available?
Verified Customer
Verified customer
Asked: 2017-10-04
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Sprouty-4 antibody A04343 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-10-04
Question
We are currently using anti-Sprouty-4 antibody A04343 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2017-06-22
Answer
The anti-Sprouty-4 antibody (A04343) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-06-22
Question
I was wanting to use your anti-Sprouty-4 antibody for IHC for human brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human brain identification?
C. Bhatt
Verified customer
Asked: 2014-10-16
Answer
It shows on the product datasheet, A04343 anti-Sprouty-4 antibody has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-10-16
