KGF Keratinocye Growth Factor Mouse Recombinant Protein

Product Name

KGF Keratinocye Growth Factor Mouse Recombinant Protein

View all KGF/FGF-7 recombinant proteins

SKU/Catalog Number

PROTP36363

Size

2ug, 10ug, 1mg

Description

Keratinocyte Growth Factor-1 Mouse Recombinant produced in E. coli is a single, non-glycosylated, polypeptide chain containing 164 amino acids and having a molecular mass of 18.9 kDa. The FGF-7 is purified by proprietary chromatographic techniques.

Storage & Handling

Lyophilized KGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF7 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Cite This Product

KGF Keratinocye Growth Factor Mouse Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP36363)

Form

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

The protein was lyophilized from a concentrated (1mg/ml) solution containing 20mM Phosphate buffer pH-8 and 0.1M NaCl.

Purity

Greater than 95.0% as determined by: (a) Analysis by HPLC. (b) Analysis by SDS-PAGE.

Predicted MW

22.509kDa

Reconstitution

It is recommended to reconstitute the lyophilized KGF in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Biological Activity

The ED50, calculated by the dose-dependant stimulation of KGF-responsive BaF3 indicator cells (measured by 3H-thymidine uptake) is < 10ng/ml corresponding to a specific activity of 100,000 Units/mg.

Reconstitution

It is recommended to reconstitute the lyophilized KGF in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Validation Images & Assay Conditions

Gene/Protein Information For FGF7 (Source: Uniprot.org, NCBI)

Alternative Names

HBGF-7; FGF7; FGF-7; KGF; Keratinocyte growth factor; Fibroblast growth factor 7; Heparin-binding growth factor 7 FGF7 HBGF-7, KGF fibroblast growth factor 7 fibroblast growth factor 7|FGF-7|heparin-binding growth factor 7|keratinocyte growth factor

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

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