Anti-Spred3 Antibody

Product Name

Anti-Spred3 Antibody

View all Spred3 Antibodies

SKU/Catalog Number

A15231

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Spred3 Antibody (Catalog # A15231). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.

Storage & Handling

Spred3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Spred3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15231)

Host

Rabbit

Contents

Spred3 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Spred3 antibody was raised against a 16 amino acid synthetic peptide near the center of the human Spred3. The immunogen is located within amino acids 140 - 190 of Spred3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

This Spred3 antibody is predicted to have no cross-reactivity to Spred1 or Spred2.

Reactive Species

A15231 is reactive to SPRED3 in Human

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

42670 MW

Background of Spred3

Spred3 is a member of the Sprouty family, a group of proteins that act as negative regulators during development. Like Spred1 and Spred2, Spred3 contains and EVH1 domain in its amino terminus and a Sprouty-related cysteine-rich (SPR) domain in its C-terminus, but does not possess a functional c-kit binding domain (KBD). The Spred proteins have also been implicated in the negative feedback regulation of FGF signaling in embryogenesis and angiogenesis. Although Spred3 was initially reported to be expressed in brain, at least one report also indicates that Spred3 can be found in liver. At least two isoforms of Spred3 are known to exist.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A15231 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Spred3 antibody can be used for detection of Spred3 by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For SPRED3 (Source: Uniprot.org, NCBI)

Alternative Names

Eve-3, spred-3, EVE-3, Sprouty-related, EVH1 domain-containing protein 3, Spred-3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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