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Product Info Summary
| SKU: | A05056-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-CD168/HMMR Picoband® Antibody
View all RHAMM/CD168 Antibodies
SKU/Catalog Number
A05056-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD168/HMMR Picoband® Antibody catalog # A05056-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD168/HMMR Picoband® Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05056-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CD168/HMMR recombinant protein (Position: K5-D686).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A05056-1 is reactive to HMMR in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
84 kDa
Calculated molecular weight
39125 MW
Background of RHAMM/CD168
Hyaluronan-mediated motility receptor (HMMR), also known as RHAMM (Receptor for Hyaluronan Mediated Motility) is a protein which in humans is encoded by the HMMR gene. It is mapped to 5q34. The protein encoded by this gene is involved in cell motility. It is expressed in breast tissue and together with other proteins, it forms a complex with BRCA1 and BRCA2, thus is potentially associated with higher risk of breast cancer. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05056-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Positive Control
WB: human Hela whole cell, human Jurkat whole cell, human SW620 whole cell, human T-47D whole cell, human Raji whole cell
ICC/IF: U20S cell, U20S cell
FCM: U937 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CD168/HMMR using anti-CD168/HMMR antibody (A05056-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human SW620 whole cell lysates,
Lane 4: human T-47D whole cell lysates,
Lane 5: human Raji whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD168/HMMR antigen affinity purified polyclonal antibody (Catalog # A05056-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD168/HMMR at approximately 84KD. The expected band size for CD168/HMMR is at 84KD.
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Figure 2. Flow Cytometry analysis of U937 cells using anti-HMMR antibody (A05056-1).
Overlay histogram showing U937 cells stained with A05056-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HMMR Antibody (A05056-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Figure 3. IF analysis of CD168/HMMR using anti-CD168/HMMR antibody (A05056-1).
CD168/HMMR was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-CD168/HMMR Antibody (A05056-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 4. IF analysis of CD168/HMMR using anti-CD168/HMMR antibody (A05056-1).
CD168/HMMR was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-CD168/HMMR Antibody (A05056-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For HMMR (Source: Uniprot.org, NCBI)
Gene Name
HMMR
Full Name
Hyaluronan mediated motility receptor
Weight
39125 MW
Alternative Names
Hyaluronan mediated motility receptor; Intracellular hyaluronic acid-binding protein; Receptor for hyaluronan-mediated motility; CD168; HMMR; IHABP; RHAMM HMMR CD168, IHABP, RHAMM hyaluronan mediated motility receptor hyaluronan mediated motility receptor|intracellular hyaluronic acid-binding protein|receptor for hyaluronan-mediated motility
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HMMR, check out the HMMR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HMMR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD168/HMMR Picoband® Antibody (A05056-1)
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