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Product Info Summary
| SKU: | A03726 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-SPR1 GPR68 Antibody
SKU/Catalog Number
A03726
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-SPR1 GPR68 Antibody (Catalog # A03726). Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SPR1 GPR68 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03726)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human SPR1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03726 is reactive to GPR68 in Human, Mouse
Reconstitution
Calculated molecular weight
41077 MW
Background of OGR1
Proton-sensing receptor involved in pH homeostasis. May represents an osteoblastic pH sensor regulating cell-mediated responses to acidosis in bone. Mediates its action by association with G proteins that stimulates inositol phosphate (IP) production or Ca2+ mobilization. The receptor is almost silent at pH 7.8 but fully activated at pH 6.8. Function also as a metastasis suppressor gene in prostate cancer By similarity.
An S., FEBS Lett. 375:121-124(1995).
Xu Y., Genomics 35:397-402(1996).
King M.M., Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03726 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from Jurkat cells and HeLa cells, using SPR1 antibody A03726.
Protein Target Info & Infographic
Gene/Protein Information For GPR68 (Source: Uniprot.org, NCBI)
Gene Name
GPR68
Full Name
Ovarian cancer G-protein coupled receptor 1
Weight
41077 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
Ovarian cancer G-protein coupled receptor 1;OGR-1;G-protein coupled receptor 68;GPR12A;Sphingosylphosphorylcholine receptor;GPR68;OGR1; GPR68 AI2A6, GPR12A, OGR1 G protein-coupled receptor 68 ovarian cancer G-protein coupled receptor 1|ovarian cancer G protein-coupled receptor, 1|sphingosylphosphorylcholine receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GPR68, check out the GPR68 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GPR68: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SPR1 GPR68 Antibody (A03726)
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No publications found for A03726
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-SPR1 GPR68 Antibody
Question
I was wanting to use your anti-SPR1 antibody for WB for human lung on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human lung identification?
Verified Customer
Verified customer
Asked: 2020-04-27
Answer
As indicated on the product datasheet, A03726 anti-SPR1 antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-27
Question
We are currently using anti-SPR1 antibody A03726 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-24
Answer
The anti-SPR1 antibody (A03726) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-24
Question
My lab would like to test anti-SPR1 antibody A03726 on human lung for research purposes, then I may be interested in using anti-SPR1 antibody A03726 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
The products we sell, including anti-SPR1 antibody A03726, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-31
Question
Will anti-SPR1 antibody A03726 work on dog WB with tendon of biceps brachii?
Verified Customer
Verified customer
Asked: 2018-10-30
Answer
Our lab technicians have not validated anti-SPR1 antibody A03726 on dog. You can run a BLAST between dog and the immunogen sequence of anti-SPR1 antibody A03726 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog tendon of biceps brachii in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-10-30
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-SPR1 antibody A03726. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-12-25
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-12-25
Question
Is this A03726 anti-SPR1 antibody reactive to the isotypes of GPR68?
Verified Customer
Verified customer
Asked: 2017-09-06
Answer
The immunogen of A03726 anti-SPR1 antibody is Synthesized peptide derived from internal of human SPR1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-09-06
Question
Is a blocking peptide available for product anti-SPR1 antibody (A03726)?
J. Jha
Verified customer
Asked: 2013-09-16
Answer
We do provide the blocking peptide for product anti-SPR1 antibody (A03726). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-09-16
