Anti-SPACA3 Antibody Picoband™

Product Name

Anti-SPACA3 Antibody Picoband™

View all Spaca3 Antibodies

SKU/Catalog Number

A10284-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-SPACA3 Antibody Picoband™ catalog # A10284-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-SPACA3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10284-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human SPACA3 recombinant protein (Position: E86-F215).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A10284-1 is reactive to Spaca3 in Human, Mouse, Rat

Applications

A10284-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

40 kDa

Calculated molecular weight

41409 MW

Background of Spaca3

Sperm acrosome membrane-associated protein 3 is a protein that in humans is encoded by the SPACA3 gene. The protein encoded by this gene is a sperm surface protein that may be involved in adhesion to the egg prior to fertilization. While the encoded protein has significant similarity to lysozyme at the amino acid level, it has no detectable bacteriocidal activity. Several transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of SPACA3 using anti-SPACA3 antibody (A10284-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Daudi whole cell lysates,
Lane 3: rat C6 whole cell lysates,
Lane 4: mouse NIH/3T3 whole cell lysates,
Lane 5: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPACA3 antigen affinity purified polyclonal antibody (Catalog # A10284-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPACA3 at approximately 40 kDa. The expected band size for SPACA3 is at 23 kDa.

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Figure 2. IF analysis of SPACA3 using anti-SPACA3 antibody (A10284-1).
SPACA3 was detected in an immunocytochemical section of CACO2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SPACA3 Antibody (A10284-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Figure 3. Flow Cytometry analysis of Daudi cells using anti-SPACA3 antibody (A10284-1).
Overlay histogram showing Daudi cells stained with A10284-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SPACA3 Antibody (A10284-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Gene/Protein Information For Spaca3 (Source: Uniprot.org, NCBI)

Alternative Names

CT54ALLP17Cancer/testis antigen 54; LYC3Sperm protein reactive with ASA; Lysozyme-like acrosomal sperm-specific secretory protein ALLP-17; Lysozyme-like protein 3; lysozyme-like sperm-specific secretory protein ALLP17; LYZL31700025M08Rik; SLLP1MGC119058; sperm acrosome associated 3; sperm acrosome membrane-associated protein 3; sperm lysozyme like protein 1; Sperm lysozyme-like protein 1; Sperm protein reactive with antisperm antibodies; SPRASA Spaca3|1700025M08Rik, AL, ALLP17, Lyc3, S, SLLP1, mSLLP1|sperm acrosome associated 3|sperm acrosome membrane-associated protein 3|acrosomal sperm protein|lysozyme-like protein 3|sperm c-type lysozyme-like protein|sperm lysozyme-like protein 1 precusor

For more info on Spaca3, check out the Spaca3 Infographic

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In this infographic, you will see the following information for Spaca3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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