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Product Info Summary
| SKU: | A00177S181 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-SOX9 (phospho-S181) Antibody
SKU/Catalog Number
A00177S181
Size
100ul
Form
Liquid
Description
Boster Bio Anti-SOX9 (phospho-S181) Antibody catalog # A00177S181. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SOX9 (phospho-S181) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00177S181)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phosphopeptide derived from human SOX9 around the phosphorylation site of Serine 181.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00177S181 is reactive to SOX9 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
56137 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00177S181 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
Validation Images & Assay Conditions
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Figure 2. Immunohistochemistry validation of SOX9 using Anti-SOX9 (phospho-S181) Antibody (A00177S181).
Immunohistochemistry (IHC) analyzes of p-SOX9 (S181) pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing nucleus staining. Negative control (the right)Using PBS instead of primary antibody
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Figure 3. Immunohistochemistry validation of SOX9 using Anti-SOX9 (phospho-S181) Antibody (A00177S181).
Immunohistochemistry (IHC) analyzes of p-SOX9 (S181) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.showing nucleus staining. Negative control (the right)Using PBS instead of primary antibody
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Figure 1. Western blotting validation for Anti-SOX9 (phospho-S181) Antibody A00177S181
Western blot (WB) analysis of p-SOX9 (S181) polyclonal antibody at 1:500 dilutionLane1:Hela lysate treated with PMA(100nM
Protein Target Info & Infographic
Gene/Protein Information For SOX9 (Source: Uniprot.org, NCBI)
Gene Name
SOX9
Full Name
Transcription factor SOX-9
Weight
56137 MW
Alternative Names
Transcription factor SOX-9 ;SOX9 SOX9 CMD1, CMPD1, SRA1, SRXX2, SRXY10 SRY-box transcription factor 9 transcription factor SOX-9|SRY (sex determining region Y)-box9|SRY (sex-determining region Y)-box 9 protein|SRY-box 9|SRY-related HMG-box, gene 9
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SOX9, check out the SOX9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SOX9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SOX9 (phospho-S181) Antibody (A00177S181)
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