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Product Info Summary
| SKU: | M00058 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Sonic Hedgehog SHH Rabbit Monoclonal Antibody
View all Sonic Hedgehog/Shh Antibodies
SKU/Catalog Number
M00058
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Sonic Hedgehog SHH Rabbit Monoclonal Antibody catalog # M00058. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Sonic Hedgehog SHH Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00058)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ABE-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Sonic Hedgehog
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00058 is reactive to SHH in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
66 kDa
Calculated molecular weight
49607 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00058 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Positive Control
IHC: human endometrium tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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Western blot analysis of Sonic Hedgehog in HepG2 cell lysate.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human endometrium, using Sonic Hedgehog Antibody.
Protein Target Info & Infographic
Gene/Protein Information For SHH (Source: Uniprot.org, NCBI)
Gene Name
SHH
Full Name
Sonic hedgehog protein
Weight
49607 MW
Superfamily
hedgehog family
Alternative Names
Sonic hedgehog protein;SHH;HHG-1;Sonic hedgehog protein N-product;Sonic hedgehog protein C-product;SHH; SHH HHG1, HLP3, HPE3, MCOPCB5, SMMCI, ShhNC, TPT, TPTPS sonic hedgehog signaling molecule sonic hedgehog protein|shh unprocessed N-terminal signaling and C-terminal autoprocessing domains|sonic hedgehog homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SHH, check out the SHH Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SHH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Sonic Hedgehog SHH Rabbit Monoclonal Antibody (M00058)
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No publications found for M00058
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Recommended Resources
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Sonic Hedgehog SHH Rabbit Monoclonal Antibody
Question
I am interested in using your anti-Sonic Hedgehog Rabbit Monoclonal antibody for bud outgrowth involved in lung branching studies. Has this antibody been tested with western blotting on hepg2 cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-04-10
Answer
We appreciate your inquiry. This M00058 anti-Sonic Hedgehog Rabbit Monoclonal antibody is validated on hepg2 cell lysate. It is guaranteed to work for IF, IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-04-10
Question
I have a question about product M00058, anti-Sonic Hedgehog Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00058 anti-Sonic Hedgehog Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-27
Question
We want to test anti-Sonic Hedgehog Rabbit Monoclonal antibody M00058 on human plasma for research purposes, then I may be interested in using anti-Sonic Hedgehog Rabbit Monoclonal antibody M00058 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-19
Answer
The products we sell, including anti-Sonic Hedgehog Rabbit Monoclonal antibody M00058, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-19
Question
I was wanting to use your anti-Sonic Hedgehog Rabbit Monoclonal antibody for IF for human plasma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human plasma identification?
Verified Customer
Verified customer
Asked: 2020-01-01
Answer
You can see on the product datasheet, M00058 anti-Sonic Hedgehog Rabbit Monoclonal antibody has been validated for IF, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human plasma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-01
Question
Is a blocking peptide available for product anti-Sonic Hedgehog Rabbit Monoclonal antibody (M00058)?
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
We do provide the blocking peptide for product anti-Sonic Hedgehog Rabbit Monoclonal antibody (M00058). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-11-22
Question
We are currently using anti-Sonic Hedgehog Rabbit Monoclonal antibody M00058 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
The anti-Sonic Hedgehog Rabbit Monoclonal antibody (M00058) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-30
Question
We have been able to see staining in human fetal lung. Do you have any suggestions? Is anti-Sonic Hedgehog Rabbit Monoclonal antibody supposed to stain fetal lung positively?
G. Anderson
Verified customer
Asked: 2019-08-12
Answer
Based on literature fetal lung does express SHH. Based on Uniprot.org, SHH is expressed in left adrenal gland, fetal lung, plasma, among other tissues. Regarding which tissues have SHH expression, here are a few articles citing expression in various tissues:
Fetal lung, Pubmed ID: 7590746
Plasma, Pubmed ID: 16335952
Boster Scientific Support
Answered: 2019-08-12
Question
I see that the anti-Sonic Hedgehog Rabbit Monoclonal antibody M00058 works with IF, what is the protocol used to produce the result images on the product page?
W. Wu
Verified customer
Asked: 2019-05-30
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-05-30
Question
We were content with the WB result of your anti-Sonic Hedgehog Rabbit Monoclonal antibody. However we have seen positive staining in plasma sonic hedgehog protein n-product: cell using this antibody. Is that expected? Could you tell me where is SHH supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-06-14
Answer
Based on literature, plasma does express SHH. Generally SHH expresses in sonic hedgehog protein n-product: cell. Regarding which tissues have SHH expression, here are a few articles citing expression in various tissues:
Fetal lung, Pubmed ID: 7590746
Plasma, Pubmed ID: 16335952
Boster Scientific Support
Answered: 2018-06-14
Question
I have attached the WB image, lot number and protocol we used for plasma using anti-Sonic Hedgehog Rabbit Monoclonal antibody M00058. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-06-26
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-06-26
Question
Would M00058 anti-Sonic Hedgehog Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-06-02
Answer
You can see on the product datasheet, M00058 anti-Sonic Hedgehog Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-06-02
Question
We purchased anti-Sonic Hedgehog Rabbit Monoclonal antibody for WB on fetal lung a few months ago. I am using human, and We are going to use the antibody for IHC next. My lab would like examining fetal lung as well as left adrenal gland in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
K. Miller
Verified customer
Asked: 2017-03-28
Answer
I viewed the website and datasheets of our anti-Sonic Hedgehog Rabbit Monoclonal antibody and I see that M00058 has been tested on human in both WB and IHC. Thus M00058 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-03-28
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for plasma using anti-Sonic Hedgehog Rabbit Monoclonal antibody M00058. Let me know if you need anything else.
K. Mangal
Verified customer
Asked: 2017-01-10
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-01-10
Question
Is this M00058 anti-Sonic Hedgehog Rabbit Monoclonal antibody reactive to the isotypes of SHH?
M. Williams
Verified customer
Asked: 2016-03-09
Answer
The immunogen of M00058 anti-Sonic Hedgehog Rabbit Monoclonal antibody is A synthesized peptide derived from human Sonic Hedgehog. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-03-09
Question
Will anti-Sonic Hedgehog Rabbit Monoclonal antibody M00058 work for IF with plasma?
L. Banerjee
Verified customer
Asked: 2015-07-07
Answer
According to the expression profile of plasma, SHH is highly expressed in plasma. So, it is likely that anti-Sonic Hedgehog Rabbit Monoclonal antibody M00058 will work for IF with plasma.
Boster Scientific Support
Answered: 2015-07-07
Question
Is there a BSA free version of anti-Sonic Hedgehog Rabbit Monoclonal antibody M00058 available?
D. Mitchell
Verified customer
Asked: 2014-06-18
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Sonic Hedgehog Rabbit Monoclonal antibody M00058 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-06-18
