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Product Info Summary
| SKU: | M01689 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-Somatostatin Receptor 2 SSTR2 Rabbit Monoclonal Antibody
View all Somatostatin R2/SSTR2 Antibodies
SKU/Catalog Number
M01689
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Somatostatin Receptor 2 SSTR2 Rabbit Monoclonal Antibody catalog # M01689. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Somatostatin Receptor 2 SSTR2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01689)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
CIF-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Somatostatin Receptor 2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01689 is reactive to SSTR2 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
120 kDa
Calculated molecular weight
41333 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01689 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
IP 1:50
Positive Control
WB: human K562 whole cell, human SH-SY5Y whole cell, human Jurkat whole cell, rat brain tissue, mouse brain tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SSTR2 using anti-SSTR2 antibody (M01689).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SSTR2 antigen affinity purified monoclonal antibody (Catalog # M01689) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SSTR2 at approximately 41 kDa. The expected band size for SSTR2 is at 41 kDa.
Protein Target Info & Infographic
Gene/Protein Information For SSTR2 (Source: Uniprot.org, NCBI)
Gene Name
SSTR2
Full Name
Somatostatin receptor type 2
Weight
41333 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
Somatostatin receptor type 2;SS-2-R;SS2-R;SS2R;SRIF-1;SSTR2; SSTR2 somatostatin receptor 2 somatostatin receptor type 2|SRIF-1|SS2R
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SSTR2, check out the SSTR2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SSTR2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Somatostatin Receptor 2 SSTR2 Rabbit Monoclonal Antibody (M01689)
Hello CJ!
M01689 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Li M, Wang S, Wang S, Zhang L, Wu D, Yang R, Ji A, Li Y, Wang J. Exp Ther Med. 2017 Aug;14(2):1732-1738. doi: 10.3892/etm.2017.4651. Epub 2017 Jun 22. Occludin downregulation in high glucose is regulated by SSTR2 via the VEGF/NRP1/Akt signaling pa...
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