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Product Info Summary
| SKU: | PA2174 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Solute carrier family 22 member 5/SLC22A5 Antibody Picoband®
View all OCTN2/SLC22A5 Antibodies
SKU/Catalog Number
PA2174
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Solute carrier family 22 member 5/SLC22A5 Antibody catalog # PA2174. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Solute carrier family 22 member 5/SLC22A5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2174)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse Solute carrier family 22 member 5, different from the related rat sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2174 is reactive to Slc22a5 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
200 kDa
Calculated molecular weight
62780 MW
Background of OCTN2/SLC22A5
Solute carrier family 22 (organic cation/carnitine transporter), member 5, also called SLC22A5 or OCTN2 is a membrane transport protein associated with primary carnitine deficiency. This gene is mapped to 5q31.1. Polyspecific organic cation transporters in the liver, kidney, intestine, and other organs are critical for elimination of many endogenous small organic cations as well as a wide array of drugs and environmental toxins. The encoded protein is a plasma integral membrane protein which functions both as an organic cation transporter and as a sodium-dependent high affinity carnitine transporter. The encoded protein is involved in the active cellular uptake of carnitine. Mutations in this gene are the cause of systemic primary carnitine deficiency (CDSP), an autosomal recessive disorder manifested early in life by hypoketotic hypoglycemia and acute metabolic decompensation, and later in life by skeletal myopathy or cardiomyopathy.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2174 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Positive Control
WB: Rat Kidney Tissue, Rat Skeletal Muscles Tissue, Mouse Liver Tissue, HEPA Cell, NIH3T3 Cell,
Validation Images & Assay Conditions
Click image to see more details
Anti-Solute carrier family 22 member 5 antibody, PA2174, Western blotting
Lane 1: Rat Kidney Tissue Lysate
Lane 2: Rat Skeletal Muscles Tissue Lysate
Lane 3: Mouse Liver Tissue Lysate
Lane 4: HEPA Cell Lysate
Lane 5: NIH3T3 Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For Slc22a5 (Source: Uniprot.org, NCBI)
Gene Name
Slc22a5
Full Name
Solute carrier family 22 member 5
Weight
62780 MW
Superfamily
major facilitator (TC 2.A.1) superfamily
Alternative Names
Solute carrier family 22 member 5;High-affinity sodium-dependent carnitine cotransporter;Organic cation/carnitine transporter 2;Slc22a5;Octn2; SLC22A5 CDSP, OCTN2 solute carrier family 22 member 5 solute carrier family 22 member 5|high-affinity sodium dependent carnitine cotransporter|organic cation/carnitine transporter 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Slc22a5, check out the Slc22a5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Slc22a5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Solute carrier family 22 member 5/SLC22A5 Antibody Picoband® (PA2174)
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Customer Q&As
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8 Customer Q&As for Anti-Solute carrier family 22 member 5/SLC22A5 Antibody Picoband®
Question
I have attached the WB image, lot number and protocol we used for trachea using anti-Solute carrier family 22 member 5/SLC22A5 antibody PA2174. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-25
Question
I see that the anti-Solute carrier family 22 member 5/SLC22A5 antibody PA2174 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-07
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-07
Question
Is there a BSA free version of anti-Solute carrier family 22 member 5/SLC22A5 antibody PA2174 available?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Solute carrier family 22 member 5/SLC22A5 antibody PA2174 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-25
Question
To generate the Western Blot result of this antibody as shown on your product page, how much sample was loaded? What is the primary antibody concentration?
Verified Customer
Verified customer
Asked: 2019-06-04
Answer
40 μg sample was loaded. The primary antibody concentration was 1 μg/mL.
Boster Scientific Support
Answered: 2019-06-04
Question
Is a blocking peptide available for product anti-Solute carrier family 22 member 5/SLC22A5 antibody (PA2174)?
Verified Customer
Verified customer
Asked: 2019-02-11
Answer
We do provide the blocking peptide for product anti-Solute carrier family 22 member 5/SLC22A5 antibody (PA2174). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-02-11
Question
Would PA2174 anti-Solute carrier family 22 member 5/SLC22A5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-11-01
Answer
As indicated on the product datasheet, PA2174 anti-Solute carrier family 22 member 5/SLC22A5 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-11-01
Question
I am looking for to test anti-Solute carrier family 22 member 5/SLC22A5 antibody PA2174 on rat trachea for research purposes, then I may be interested in using anti-Solute carrier family 22 member 5/SLC22A5 antibody PA2174 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-08-22
Answer
The products we sell, including anti-Solute carrier family 22 member 5/SLC22A5 antibody PA2174, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-08-22
Question
We are currently using anti-Solute carrier family 22 member 5/SLC22A5 antibody PA2174 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on dog tissues as well?
R. Bhatt
Verified customer
Asked: 2015-05-12
Answer
The anti-Solute carrier family 22 member 5/SLC22A5 antibody (PA2174) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-05-12
