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Product Info Summary
| SKU: | PA1763 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-DCI/ECI1 Antibody Picoband®
SKU/Catalog Number
PA1763
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-DCI/ECI1 Antibody catalog # PA1763. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DCI/ECI1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1763)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human DCI, different from the related mouse sequence by two amino acids and from the related rat sequence by three amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1763 is reactive to ECI1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
33 kDa
Calculated molecular weight
32816 MW
Background of DCI
ECI1/DCI (Dodecenoyl-CoA Delta Isomerase), also known as 3,2-trans-enoyl-CoA isomerase, is an enzyme that catalyzes the conversion of cis-or trans-double bonds of fatty acids at gamma-carbon (position 3) to trans double bonds at beta-carbon (position 2). It plays a particularly important role in the metabolism of unsaturated fatty acids. All classes of enoyl-CoA isomerases belong to a family of enzymes, the hydratase/isomerase or crotonase superfamily, and when examined with x-ray crystallography, exhibit a common structural feature of the family, the N-terminal core with a spiral fold composed of four turns, each turn consisting of two beta-sheets and one alpha-helix. Dodecenoyl-CoA Delta Isomerase is involved in the beta-oxidation, one of the most frequently used pathways in fatty acid degradation, of unsaturated fatty acids with double bonds at odd-numbered carbon positions. It does so by shifting the position of the double bonds in the acyl-CoA intermediates and converting 3-cis or trans-enoyl-CoA to 2-trans-enoyl-CoA.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1763 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunofluorescence, 2μg/ml, Human
Positive Control
WB: Rat Liver Tissue, Human Placenta Tissue, A549 Whole Cell, SMMC Whole Cell, COLO320 Whole Cell, HELA Whole Cell, HT1080 Whole Cell,
IHC: human mammary cancer tissue
ICC/IF: A549 cell, A549 cell
ICC: human Hela cell, human A549 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of DCI using anti-DCI antibody (PA1763).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Rat Liver Tissue Lysate,
Lane 2: Human Placenta Tissue Lysate,
Lane 3: A549 Whole Cell Lysate,
Lane 4: SMMC Whole Cell Lysate,
Lane 5: COLO320 Whole Cell Lysate,
Lane 6: HELA Whole Cell Lysate,
Lane 7: HT1080 Whole Cell Lysate,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DCI antigen affinity purified polyclonal antibody (Catalog # PA1763) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DCI at approximately 33KD. The expected band size for DCI is at 33KD.
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Figure 2. IHC analysis of DCI using anti-DCI antibody (PA1763).
DCI was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DCI Antibody (PA1763) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of DCI using anti-DCI antibody (PA1763).
DCI was detected in immunocytochemical section of human Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-DCI Antibody (PA1763) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of DCI using anti-DCI antibody (PA1763).
DCI was detected in immunocytochemical section of human A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-DCI Antibody (PA1763) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 5. IF analysis of DCI using anti-DCI antibody (PA1763).
DCI was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-DCI Antibody (PA1763) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 6. IF analysis of DCI using anti-DCI antibody (PA1763).
DCI was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-DCI Antibody (PA1763) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For ECI1 (Source: Uniprot.org, NCBI)
Gene Name
ECI1
Full Name
Enoyl-CoA delta isomerase 1, mitochondrial
Weight
32816 MW
Superfamily
enoyl-CoA hydratase/isomerase family
Alternative Names
Enoyl-CoA delta isomerase 1, mitochondrial;5.3.3.8;3,2-trans-enoyl-CoA isomerase;Delta (3),Delta (2)-enoyl-CoA isomerase;D3,D2-enoyl-CoA isomerase;Dodecenoyl-CoA isomerase;ECI1;DCI; ECI1 DCI enoyl-CoA delta isomerase 1 enoyl-CoA delta isomerase 1, mitochondrial|3,2 trans-enoyl-Coenzyme A isomerase|3,2-trans-enoyl-CoA isomerase, mitochondrial|D3,D2-enoyl-CoA isomerase|acetylene-allene isomerase|delta(3),Delta(2)-enoyl-CoA isomerase|dodecenoyl-CoA delta isomerase (3,2 trans-enoyl-CoA isomerase)|dodecenoyl-Coenzyme A delta isomerase (3,2 trans-enoyl-Coenzyme A isomerase)|epididymis secretory sperm binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ECI1, check out the ECI1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ECI1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DCI/ECI1 Antibody Picoband® (PA1763)
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Customer Q&As
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5 Customer Q&As for Anti-DCI/ECI1 Antibody Picoband®
Question
Would anti-DCI/ECI1 antibody PA1763 work for ICC with lung placenta?
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
According to the expression profile of lung placenta, ECI1 is highly expressed in lung placenta. So, it is likely that anti-DCI/ECI1 antibody PA1763 will work for ICC with lung placenta.
Boster Scientific Support
Answered: 2019-12-04
Question
Is this PA1763 anti-DCI/ECI1 antibody reactive to the isotypes of ECI1?
Verified Customer
Verified customer
Asked: 2019-02-04
Answer
The immunogen of PA1763 anti-DCI/ECI1 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human DCI(272-290aa ADVQNFVSFISKDSIQKSL), different from the related mouse sequence by two amino acids and from the related rat sequence by three amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-02-04
Question
Is there a BSA free version of anti-DCI/ECI1 antibody PA1763 available?
T. Edwards
Verified customer
Asked: 2018-08-01
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-DCI/ECI1 antibody PA1763 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-08-01
Question
We are currently using anti-DCI/ECI1 antibody PA1763 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
B. Kulkarni
Verified customer
Asked: 2014-10-16
Answer
The anti-DCI/ECI1 antibody (PA1763) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-10-16
Question
I was wanting to use your anti-DCI/ECI1 antibody for ICC for human lung placenta on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human lung placenta identification?
R. Gonzalez
Verified customer
Asked: 2014-10-16
Answer
As indicated on the product datasheet, PA1763 anti-DCI/ECI1 antibody has been tested for IF, IHC-P, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human lung placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-10-16
