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Product Info Summary
| SKU: | M01784 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Guinea pig, Human, Mouse, Rat |
| Host: | Mouse |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-SOD (EC) Monoclonal Antibody
View all SOD3/EC-SOD Antibodies
SKU/Catalog Number
M01784
Size
100ug
Form
liquid
Description
Boster Bio Anti-SOD (EC) Monoclonal Antibody catalog # M01784. Tested in ELISA, IF, ICC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SOD (EC) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01784)
Host
Mouse
Contents
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Clonality
Monoclonal
Clone Number
4GG11G6
Isotype
IgG1 Kappa
Immunogen
Human extracellular SOD purified from aortas
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects extracellular SOD ~35kDa.
Reactive Species
M01784 is reactive to SOD3 in Guinea pig, Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
62081 MW
Background of SOD3/EC-SOD
Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2, 5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are three types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (6). SOD3 can also be distinguished by its heparin-binding capacity (1).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01784 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB (1:1000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Validation Images & Assay Conditions
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Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SOD3 Monoclonal Antibody, Clone 4GG11G6 (M01784) . Tissue: cartilage. Species: Human. Primary Antibody: Mouse Anti-SOD3 Monoclonal Antibody (M01784) at 1:1000. Left: control, middle: young cartilage, right: cartilage with osteoarthritis..
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Figure 2. Western blot analysis of SOD3 using anti-SOD3 antibody (M01784).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOD3 antigen affinity purified polyclonal antibody (Catalog # M01784) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1021) with Tanon 5200 system. A specific band was detected for SOD3.
Protein Target Info & Infographic
Gene/Protein Information For SOD3 (Source: Uniprot.org, NCBI)
Gene Name
SOD3
Full Name
Extracellular superoxide dismutase [Cu-Zn]
Weight
62081 MW
Superfamily
Cu-Zn superoxide dismutase family
Alternative Names
EC SOD, EC-SOD, Extracellular superoxide dismutase [Cu Zn], Extracellular superoxide dismutase [Cu-Zn], Extracellular superoxide dismutase, Extracellular superoxide dismutase precursor, MGC20077, SOD 3, SOD3, SODE_HUMAN, Superoxide dismutase 3 extracellular SOD3 EC-SOD superoxide dismutase 3 extracellular superoxide dismutase [Cu-Zn]|superoxide dismutase 3, extracellular|testicular tissue protein Li 175
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SOD3, check out the SOD3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SOD3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SOD (EC) Monoclonal Antibody (M01784)
Hello CJ!
M01784 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Epigallocatechin%u20103%u2010Gallate Attenuates Oxidative Stress and Inflammation in Obstructive Nephropathy via NF%u2010%u03BAB and Nrf2/HO%u20101 Signalling Pathway Regulation
Long Sh, Yu Zq, Shuai L, Guo Yl, Duan Dl, Xu Xy, Li Xd. Int J Mol Sci. 2012;13(3):3354-65. Doi: 10.3390/Ijms13033354. Epub 2012 Mar 12. The Hypoglycemic Effect Of The Kelp On Diabetes Mellitus Model Induced By Alloxan In Rats.
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