Product Info Summary
SKU: | A11463-2 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-SNX15 Antibody Picoband™
SKU/Catalog Number
A11463-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SNX15 Antibody Picoband™ catalog # A11463-2. Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SNX15 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11463-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SNX15 recombinant protein (Position: D8-K327).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A11463-2 is reactive to SNX15 in Human
Applications
A11463-2 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
50 kDa
Calculated molecular weight
Background of SNX15
Sorting nexin-15 is a protein that in humans is encoded by the SNX15 gene. This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. Overexpression of this gene results in a decrease in the processing of insulin and hepatocyte growth factor receptors to their mature subunits. This decrease is caused by the mislocalization of furin, the endoprotease responsible for cleavage of insulin and hepatocyte growth factor receptors. This protein is involved in endosomal trafficking from the plasma membrane to recycling endosomes or the trans-Golgi network. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ADP-ribosylation factor-like 2 (ARL2) gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human
Direct ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
![a11463 2 snx15 primary antibodies wb testing 1 a11463 2 snx15 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/1/a11463-2-snx15-primary-antibodies-wb-testing-1.jpg)
Click image to see more details
Figure 1. Western blot analysis of SNX15 using anti-SNX15 antibody (A11463-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNX15 antigen affinity purified polyclonal antibody (Catalog # A11463-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNX15 at approximately 50 kDa. The expected band size for SNX15 is at 38 kDa.
Protein Target Info & Infographic
Gene/Protein Information For SNX15 (Source: Uniprot.org, NCBI)
Gene Name
SNX15
Full Name
Sorting nexin-15
Weight
Superfamily
sorting nexin family
Alternative Names
clone iota unknown protein; HSAF001435; sorting nexin 15; sorting nexin-15 SNX15 HSAF001435 sorting nexin 15 sorting nexin-15|clone iota unknown protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SNX15, check out the SNX15 Infographic
![SNX15 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SNX15: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SNX15 Antibody Picoband™ (A11463-2)
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