Anti-SNAI3 Antibody Picoband™

Product Name

Anti-SNAI3 Antibody Picoband™

View all Snai3 Antibodies

SKU/Catalog Number

PB9440

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SNAI3 Antibody Picoband™ catalog # PB9440. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SNAI3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9440)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human SNAI3, different from the related mouse sequence by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9440 is reactive to Snai3 in Human

Applications

PB9440 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

32 kDa

Calculated molecular weight

32474 MW

Background of Snai3

SNAI3 is a member of the SNAIL gene family, named for the Drosophila snail gene, which plays roles in mesodermal formation during embryogenesis. SNAI3 gene, was located between KIAA0233 gene and CBFA2T3 gene in human chromosome 16q24.3, a region affected in breast cancer, gastric cancer, hepatocellular carcinoma, ovarian cancer, and therapy-related myeloid leukemia with t (16;21) (q24;q22) translocation. Human SNAI3 gene was found to encode 292-amino-acid polypeptide with the N-terminal SNAG domain and five zinc finger domains. And Human SNAI3 mRNA was expressed in skin melanotic melanoma, lung epidermoid carcinoma, and germ cell tumor. Because SNAG zinc-finger proteins are transcriptional repressors implicated in carcinogenesis and embryogenesis, SNAI3 gene might be a potent target of pharmacogenomics in the field of oncology and regenerative medicine.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of SNAI3 using anti-SNAI3 antibody (PB9440).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: MCF-7 Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNAI3 antigen affinity purified polyclonal antibody (Catalog # PB9440) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNAI3 at approximately 32 kDa. The expected band size for SNAI3 is at 32 kDa.

Gene/Protein Information For Snai3 (Source: Uniprot.org, NCBI)

Alternative Names

MGC129606; protein snail homolog 3; SMUC; snail homolog 3 (Drosophila); SNAIL3; Zfp293; zinc finger protein 293; zinc finger protein SNAI3; ZNF293 Snai3|AI643946, Smu, Smuc, Zfp29, Zfp293|snail family zinc finger 3|zinc finger protein SNAI3|protein snail homolog 3|snail homolog 3|snail-related gene from muscle cells|snail-related protein 2|zinc finger protein 293

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In this infographic, you will see the following information for Snai3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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