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Product Info Summary
| SKU: | PB9440 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-SNAI3 Antibody Picoband®
SKU/Catalog Number
PB9440
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SNAI3 Antibody Picoband® catalog # PB9440. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SNAI3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9440)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human SNAI3, different from the related mouse sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9440 is reactive to SNAI3 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
32 kDa
Calculated molecular weight
32474 MW
Background of Snai3
SNAI3 is a member of the SNAIL gene family, named for the Drosophila snail gene, which plays roles in mesodermal formation during embryogenesis. SNAI3 gene, was located between KIAA0233 gene and CBFA2T3 gene in human chromosome 16q24.3, a region affected in breast cancer, gastric cancer, hepatocellular carcinoma, ovarian cancer, and therapy-related myeloid leukemia with t (16;21) (q24;q22) translocation. Human SNAI3 gene was found to encode 292-amino-acid polypeptide with the N-terminal SNAG domain and five zinc finger domains. And Human SNAI3 mRNA was expressed in skin melanotic melanoma, lung epidermoid carcinoma, and germ cell tumor. Because SNAG zinc-finger proteins are transcriptional repressors implicated in carcinogenesis and embryogenesis, SNAI3 gene might be a potent target of pharmacogenomics in the field of oncology and regenerative medicine.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9440 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: MCF-7 Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of SNAI3 using anti-SNAI3 antibody (PB9440).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: MCF-7 Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNAI3 antigen affinity purified polyclonal antibody (Catalog # PB9440) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNAI3 at approximately 32 kDa. The expected band size for SNAI3 is at 32 kDa.
Protein Target Info & Infographic
Gene/Protein Information For SNAI3 (Source: Uniprot.org, NCBI)
Gene Name
SNAI3
Full Name
Zinc finger protein SNAI3
Weight
32474 MW
Superfamily
snail C2H2-type zinc-finger protein family
Alternative Names
Zinc finger protein SNAI3;Protein snail homolog 3;Zinc finger protein 293;SNAI3;ZNF293; Snai3|AI643946, Smu, Smuc, Zfp29, Zfp293|snail family zinc finger 3|zinc finger protein SNAI3|protein snail homolog 3|snail homolog 3|snail-related gene from muscle cells|snail-related protein 2|zinc finger protein 293
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SNAI3, check out the SNAI3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SNAI3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SNAI3 Antibody Picoband® (PB9440)
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Customer Q&As
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5 Customer Q&As for Anti-SNAI3 Antibody Picoband®
Question
We are currently using anti-SNAI3 antibody PB9440 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
The anti-SNAI3 antibody (PB9440) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-14
Question
I was wanting to use your anti-SNAI3 antibody for WB for human lung on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human lung identification?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
You can see on the product datasheet, PB9440 anti-SNAI3 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-08
Question
Is a blocking peptide available for product anti-SNAI3 antibody (PB9440)?
R. Roberts
Verified customer
Asked: 2019-05-21
Answer
We do provide the blocking peptide for product anti-SNAI3 antibody (PB9440). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-05-21
Question
Does anti-SNAI3 antibody PB9440 work on monkey WB with lung?
Verified Customer
Verified customer
Asked: 2019-05-14
Answer
Our lab technicians have not tested anti-SNAI3 antibody PB9440 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-SNAI3 antibody PB9440 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey lung in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-14
Question
I see that the anti-SNAI3 antibody PB9440 works with WB, what is the protocol used to produce the result images on the product page?
B. Walker
Verified customer
Asked: 2016-07-22
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-07-22
