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Product Info Summary
| SKU: | M02422 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Chicken, Cow, Dog, Guinea pig, Human, Pig, Rabbit, Rat, Cat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC |
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Product info
Product Name
Anti-Smooth Muscle Myosin Heavy Chain (SM-MHC) (Leiomyosarcoma & Myoepithelial Cell Marker) MYH11 Monoclonal Antibody
View all Myosin heavy chain 11 Antibodies
SKU/Catalog Number
M02422
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-Smooth Muscle Myosin Heavy Chain (SM-MHC) (Leiomyosarcoma & Myoepithelial Cell Marker) MYH11 Monoclonal Antibody (Catalog # M02422). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Cow, Pig, Dog, Cat, Rabbit, Rat, Guinea pig, Chicken.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-Smooth Muscle Myosin Heavy Chain (SM-MHC) (Leiomyosarcoma & Myoepithelial Cell Marker) MYH11 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02422)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: ID8
Isotype
IgG1, kappa
Immunogen
Human uterus extract
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M02422 is reactive to MYH11 in Chicken, Cow, Dog, Guinea pig, Human, Pig, Rabbit, Rat, Cat
Reconstitution
Reconstitute with distilled water.
Observed Molecular Weight
68 kDa
Calculated molecular weight
227339 MW
Background of Myosin heavy chain 11
Smooth muscle myosin heavy chain (SM-MHC) is a cytoplasmic structural protein, which is a major component of the contractile apparatus in smooth muscle cells. Expression of smooth muscle myosin is developmentally regulated, appearing early in smooth muscle development, and is specific for smooth muscle development. Two isoforms of smooth muscle myosin heavy chain have been identified, designated MHC-1 and MHC-2. The antibody may be useful for the study of breast tumors as the presence of an intact layer of myoepithelial cells is an important feature, which may distinguish benign breast lesions and carcinoma in situ from invasive tumors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02422 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Anti-SM-MHC Monoclonal Antibody (ID8).
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Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with Anti-SM-MHC Monoclonal Antibody (ID8).
Protein Target Info & Infographic
Gene/Protein Information For MYH11 (Source: Uniprot.org, NCBI)
Gene Name
MYH11
Full Name
Myosin-11
Weight
227339 MW
Superfamily
TRAFAC class myosin-kinesin ATPase superfamily
Alternative Names
Myosin-11;Myosin heavy chain 11;Myosin heavy chain, smooth muscle isoform;SMMHC;MYH11;KIAA0866; MYH11 AAT4, FAA4, SMHC, SMMHC myosin heavy chain 11 myosin-11|epididymis secretory sperm binding protein|myosin, heavy chain 11, smooth muscle|myosin, heavy polypeptide 11, smooth muscle
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MYH11, check out the MYH11 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MYH11: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Smooth Muscle Myosin Heavy Chain (SM-MHC) (Leiomyosarcoma & Myoepithelial Cell Marker) MYH11 Monoclonal Antibody (M02422)
Hello CJ!
M02422 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The penetration and phenotype modulation of smooth muscle cells on surface heparin modified poly(ɛ‐caprolactone) vascular scaffold
All-trans retinoic acid promotes smooth muscle cell differentiation of rabbit bone marrow-derived mesenchymal stem cells\n
All-trans retinoic acid promotes smooth muscle cell differentiation of rabbit bone marrow-derived mesenchymal stem cells\n
Cao, J., Geng, X., Wen, J., Li, Q., Ye, L., Zhang, A.,…, & Gu, Y. (2017). The penetration and phenotype modulation of smooth muscle cells on surface heparin modified poly(ɛ-caprolactone) vascular scaffold. Journal of Biomedical Materials Research....
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Smooth Muscle Myosin Heavy Chain (SM-MHC) (Leiomyosarcoma & Myoepithelial Cell Marker) MYH11 Monoclonal Antibody
Question
Please see the WB image, lot number and protocol we used for hippocampus using anti-Smooth Muscle Myosin Heavy Chain (SM-MHC) (Leiomyosarcoma & Myoepithelial Cell Marker) Monoclonal antibody M02422. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-16
Question
I see that the anti-Smooth Muscle Myosin Heavy Chain (SM-MHC) (Leiomyosarcoma & Myoepithelial Cell Marker) Monoclonal antibody M02422 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-19
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-19
Question
Would anti-Smooth Muscle Myosin Heavy Chain (SM-MHC) (Leiomyosarcoma & Myoepithelial Cell Marker) Monoclonal antibody M02422 work on pig IHC with liver?
Verified Customer
Verified customer
Asked: 2019-06-10
Answer
Our lab technicians have not tested anti-Smooth Muscle Myosin Heavy Chain (SM-MHC) (Leiomyosarcoma & Myoepithelial Cell Marker) Monoclonal antibody M02422 on pig. You can run a BLAST between pig and the immunogen sequence of anti-Smooth Muscle Myosin Heavy Chain (SM-MHC) (Leiomyosarcoma & Myoepithelial Cell Marker) Monoclonal antibody M02422 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig liver in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-10
Question
I was wanting to use your anti-Smooth Muscle Myosin Heavy Chain (SM-MHC) (Leiomyosarcoma & Myoepithelial Cell Marker) Monoclonal antibody for IHC for guinea pig hippocampus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for guinea pig hippocampus identification?
Verified Customer
Verified customer
Asked: 2019-05-06
Answer
As indicated on the product datasheet, M02422 anti-Smooth Muscle Myosin Heavy Chain (SM-MHC) (Leiomyosarcoma & Myoepithelial Cell Marker) Monoclonal antibody has been validated for IHC on bovine, canine, chicken, guinea pig, human, pig, rabbit, rat tissues. We have an innovator award program that if you test this antibody and show it works in guinea pig hippocampus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-06
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for hippocampus using anti-Smooth Muscle Myosin Heavy Chain (SM-MHC) (Leiomyosarcoma & Myoepithelial Cell Marker) Monoclonal antibody M02422. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-03-04
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-03-04
Question
Will anti-Smooth Muscle Myosin Heavy Chain (SM-MHC) (Leiomyosarcoma & Myoepithelial Cell Marker) Monoclonal antibody M02422 work for IHC with hippocampus?
P. Carter
Verified customer
Asked: 2018-05-29
Answer
According to the expression profile of hippocampus, MYH11 is highly expressed in hippocampus. So, it is likely that anti-Smooth Muscle Myosin Heavy Chain (SM-MHC) (Leiomyosarcoma & Myoepithelial Cell Marker) Monoclonal antibody M02422 will work for IHC with hippocampus.
Boster Scientific Support
Answered: 2018-05-29
Question
Will M02422 anti-Smooth Muscle Myosin Heavy Chain (SM-MHC) (Leiomyosarcoma & Myoepithelial Cell Marker) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-05-24
Answer
As indicated on the product datasheet, M02422 anti-Smooth Muscle Myosin Heavy Chain (SM-MHC) (Leiomyosarcoma & Myoepithelial Cell Marker) Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-05-24
