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Product Info Summary
| SKU: | PA2212 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Matrix metalloproteinase-24 MMP24 Antibody Picoband®
View all MMP-24/MT5-MMP Antibodies
SKU/Catalog Number
PA2212
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Matrix metalloproteinase-24 MMP24 Antibody catalog # PA2212. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Matrix metalloproteinase-24 MMP24 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2212)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human MMP24, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2212 is reactive to MMP24 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
47 kDa
Calculated molecular weight
73231 MW
Background of MMP-24/MT5-MMP
Matrix metalloproteinase-24, also known as MT5MMP or MMP25, is an enzyme that in humans is encoded by the MMP24 gene. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 by cleavage. The gene has previously been referred to as MMP25 but has been renamed MMP24. This gene may be a proteoglycanase involved in degradation of proteoglycans, such as dermatan sulfate and chondroitin sulfate proteoglycans.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2212 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: PANC Cell, HELA Cell, SMMC Cell, A549 Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-MMP24 antibody, PA2212, Western blotting
Lane 1: PANC Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: SMMC Cell Lysate
Lane 4: A549 Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For MMP24 (Source: Uniprot.org, NCBI)
Gene Name
MMP24
Full Name
Matrix metalloproteinase-24
Weight
73231 MW
Superfamily
peptidase M10A family
Alternative Names
Matrix metalloproteinase-24;MMP-24;3.4.24.-;Membrane-type matrix metalloproteinase 5;MT-MMP 5;MTMMP5;Membrane-type-5 matrix metalloproteinase;MT5-MMP;MT5MMP;Processed matrix metalloproteinase-24;MMP24;MT5MMP; MMP24 MMP-24, MMP25, MT-MMP 5, MT-MMP5, MT5-MMP, MT5MMP, MTMMP5 matrix metallopeptidase 24 matrix metalloproteinase-24|matrix metallopeptidase 24 (membrane-inserted)|matrix metalloproteinase 24 (membrane-inserted)|membrane-type 5 matrix metalloproteinase|membrane-type matrix metalloproteinase 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MMP24, check out the MMP24 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MMP24: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Matrix metalloproteinase-24 MMP24 Antibody Picoband® (PA2212)
Hello CJ!
PA2212 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Liu M, Liu S, Tan W, Tang F, Long J, Li Z, Liang B, Chu C, Yang J. Mol Med Rep. 2017 Dec;16(6):8953-8963. doi: 10.3892/mmr.2017.7714. Epub 2017 Oct 4. Gaseous signalling molecule SO2 via Hippo-MST pathway to improve myocardial fibrosis of diabetic...
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Customer Q&As
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4 Customer Q&As for Anti-Matrix metalloproteinase-24 MMP24 Antibody Picoband®
Question
I see that the anti-MMP24 antibody PA2212 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-03
Question
Would anti-MMP24 antibody PA2212 work on zebrafish WB with right hemisphere of cerebellum?
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
Our lab technicians have not validated anti-MMP24 antibody PA2212 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-MMP24 antibody PA2212 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish right hemisphere of cerebellum in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-02
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-MMP24 antibody PA2212. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-04-13
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-04-13
Question
We are currently using anti-MMP24 antibody PA2212 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?
D. Bhatt
Verified customer
Asked: 2014-06-05
Answer
The anti-MMP24 antibody (PA2212) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-06-05
