Anti-SMARCD3 Antibody (N-term)

Product Name

Anti-SMARCD3 Antibody (N-term)

View all SMARCD3 Antibodies

SKU/Catalog Number

A06464

Size

80 µl

Description

Boster Bio Anti-SMARCD3 Antibody (N-term) (Catalog # A06464). Tested in IF, WB application(s). This antibody reacts with Human.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-SMARCD3 Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06464)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

This SMARCD3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 27-60 amino acids from the N-terminal region of human SMARCD3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A06464 is reactive to SMARCD3 in Human

Applications

A06464 is guaranteed for IF, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

55016 Da

Background of SMARCD3

Plays a role in ATP dependent nucleosome remodeling by SMARCA4 containing complexes. Stimulates nuclear receptor mediated transcription. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron- specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post- mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IF: 1:25
WB: 1:1000

Validation Images & Assay Conditions

Click image to see more details

Fluorescent image of Hela cells stained with SMARCD3 Antibody (N-term). A06464 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).

Click image to see more details

Western blot analysis of lysate from human heart tissue lysate, using SMARCD3 Antibody (N-term) (Cat. #A06464). A06464 was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

Gene/Protein Information For SMARCD3 (Source: Uniprot.org, NCBI)

Alternative Names

60 kDa BRG-1/Brm-associated factor subunit C; 60kDa BRG-1/Brm associated factor subunit c; BAF60Cchromatin remodeling complex BAF60C subunit; BRG1-associated factor 60C; CRACD3; mammalian chromatin remodeling complex BRG1-associated factor 60C; MGC111010; Rsc6p; subfamily d, member 3; SWI/SNF complex 60 kDa subunit C; SWI/SNF related, matrix associated, actin dependent regulator of chromatin; SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily D member 3; Swp73-like protein SMARCD3 BAF60C, CRACD3, Rsc6p SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 3 SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 3|60 kDa BRG-1/Brm-associated factor subunit C|BRG1-associated factor 60C|SWI/SNF complex 60 kDa subunit C|Swp73-like protein|chromatin remodeling complex BAF60C subunit|mammalian chromatin remodeling complex BRG1-associated factor 60C

For more info on SMARCD3, check out the SMARCD3 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SMARCD3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the SMARCD3 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis