Click image to see more details
Product Info Summary
| SKU: | A03303-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-SMARCC1/BAF155 Antibody Picoband®
SKU/Catalog Number
A03303-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SMARCC1/BAF155 Antibody Picoband® catalog # A03303-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SMARCC1/BAF155 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03303-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SMARCC1/BAF155 recombinant protein (Position: K61-H95).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03303-3 is reactive to SMARCC1 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
160 kDa
Calculated molecular weight
122.867kDa
Background of SMARCC1
SWI/SNF complex subunit SMARCC1 is a protein that in humans is encoded by the SMARCC1 gene. The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03303-3 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Hela whole cell, human K562 whole cell, human Jurkat whole cell, human Hel whole cell, human Raji whole cell
FCM: K562 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of SMARCC1/BAF155 using anti-SMARCC1/BAF155 antibody (A03303-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human Hel whole cell lysates,
Lane 5: human Raji whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMARCC1/BAF155 antigen affinity purified polyclonal antibody (Catalog # A03303-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMARCC1/BAF155 at approximately 160 kDa. The expected band size for SMARCC1/BAF155 is at 160 kDa.
Click image to see more details
Figure 2. Flow Cytometry analysis of K562 cells using anti-SMARCC1/BAF155 antibody (A03303-3).
Overlay histogram showing K562 cells stained with A03303-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SMARCC1/BAF155 Antibody (A03303-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For SMARCC1 (Source: Uniprot.org, NCBI)
Gene Name
SMARCC1
Full Name
SWI/SNF complex subunit SMARCC1
Weight
122.867kDa
Superfamily
SMARCC family
Alternative Names
Glutathione S-transferase Mu 3; GST class-mu 3; GSTM3-3; hGSTM3-3; GSTM3; GST5; SMARCC1 BAF155, CRACC1, Rsc8, SRG3, SWI3 SWI/SNF related, matrix associated, actin dependent regulator of chromatin subfamily c member 1 SWI/SNF complex subunit SMARCC1|BRG1-associated factor 155|SWI/SNF complex 155 kDa subunit|SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1|chromatin remodeling complex BAF155 subunit|mammalian chromatin remodeling complex BRG1-associated factor 155
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SMARCC1, check out the SMARCC1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SMARCC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SMARCC1/BAF155 Antibody Picoband® (A03303-3)
Hello CJ!
No publications found for A03303-3
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-SMARCC1/BAF155 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-SMARCC1/BAF155 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
