Anti-SMARCC1/BAF155 Antibody Picoband™

Product Name

Anti-SMARCC1/BAF155 Antibody Picoband™

View all SMARCC1 Antibodies

SKU/Catalog Number

A03303-3

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SMARCC1/BAF155 Antibody Picoband™ catalog # A03303-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-SMARCC1/BAF155 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03303-3)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human SMARCC1/BAF155 recombinant protein (Position: K61-H95).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A03303-3 is reactive to SMARCC1 in Human

Applications

A03303-3 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

160 kDa

Calculated molecular weight

Background of SMARCC1

SWI/SNF complex subunit SMARCC1 is a protein that in humans is encoded by the SMARCC1 gene. The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of SMARCC1/BAF155 using anti-SMARCC1/BAF155 antibody (A03303-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human Hel whole cell lysates,
Lane 5: human Raji whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMARCC1/BAF155 antigen affinity purified polyclonal antibody (Catalog # A03303-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMARCC1/BAF155 at approximately 160 kDa. The expected band size for SMARCC1/BAF155 is at 160 kDa.

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Figure 2. Flow Cytometry analysis of K562 cells using anti-SMARCC1/BAF155 antibody (A03303-3).
Overlay histogram showing K562 cells stained with A03303-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SMARCC1/BAF155 Antibody (A03303-3, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Gene/Protein Information For SMARCC1 (Source: Uniprot.org, NCBI)

Alternative Names

BAF155SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily C member 1; BRG1-associated factor 155; chromatin remodeling complex BAF155 subunit; CRACC1; mammalian chromatin remodeling complex BRG1-associated factor 155; Rsc8; SRG3; subfamily c, member 1; SWI/SNF complex 155 kDa subunit; SWI/SNF complex subunit SMARCC1; SWI/SNF related, matrix associated, actin dependent regulator of chromatin; SWI3 SMARCC1 BAF155, CRACC1, Rsc8, SRG3, SWI3 SWI/SNF related, matrix associated, actin dependent regulator of chromatin subfamily c member 1 SWI/SNF complex subunit SMARCC1|BRG1-associated factor 155|SWI/SNF complex 155 kDa subunit|SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1|chromatin remodeling complex BAF155 subunit|mammalian chromatin remodeling complex BRG1-associated factor 155

For more info on SMARCC1, check out the SMARCC1 Infographic

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In this infographic, you will see the following information for SMARCC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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