Anti-(Mouse) Smarcc1 Antibody (C-term)

Product Name

Anti-(Mouse) Smarcc1 Antibody (C-term)

View all SMARCC1 Antibodies

SKU/Catalog Number

A03303-2

Size

80 µl

Description

Boster Bio Anti-(Mouse) Smarcc1 Antibody (C-term) (Catalog # A03303-2). Tested in IF, WB application(s). This antibody reacts with Human, Mouse.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-(Mouse) Smarcc1 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03303-2)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

This (Mouse) Smarcc1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 963-997 amino acids from the C-terminal region of (Mouse) Smarcc1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A03303-2 is reactive to SMARCC1 in Human, Mouse

Applications

A03303-2 is guaranteed for IF, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

122890 Da

Background of SMARCC1

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). May stimulate the ATPase activity of the catalytic subunit of the complex. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR- mediated transrepression of the CYP27B1 gene (By similarity). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron- specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IF: 1:25
WB: 1:1000

Validation Images & Assay Conditions

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Fluorescent image of Hela cells stained with (Mouse) Smarcc1 Antibody (C-term). A03303-2 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).

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Western blot analysis of lysates from Hela, mouse C2C12 cell line (from left to right), using Smarcc1 Antibody (C-term). A03303-2 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.

Gene/Protein Information For SMARCC1 (Source: Uniprot.org, NCBI)

Alternative Names

BAF155SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily C member 1; BRG1-associated factor 155; chromatin remodeling complex BAF155 subunit; CRACC1; mammalian chromatin remodeling complex BRG1-associated factor 155; Rsc8; SRG3; subfamily c, member 1; SWI/SNF complex 155 kDa subunit; SWI/SNF complex subunit SMARCC1; SWI/SNF related, matrix associated, actin dependent regulator of chromatin; SWI3 SMARCC1 BAF155, CRACC1, Rsc8, SRG3, SWI3 SWI/SNF related, matrix associated, actin dependent regulator of chromatin subfamily c member 1 SWI/SNF complex subunit SMARCC1|BRG1-associated factor 155|SWI/SNF complex 155 kDa subunit|SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1|chromatin remodeling complex BAF155 subunit|mammalian chromatin remodeling complex BRG1-associated factor 155

For more info on SMARCC1, check out the SMARCC1 Infographic

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In this infographic, you will see the following information for SMARCC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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