Click image to see more details
Product Info Summary
| SKU: | M00728 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Smad1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00728
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Smad1 Rabbit Monoclonal Antibody catalog # M00728. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Smad1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00728)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
HCH-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Smad1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00728 is reactive to SMAD1 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
90 kDa
Calculated molecular weight
52260 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00728 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Positive Control
WB: 293T cell
IHC: human colon tissue
Validation Images & Assay Conditions
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Western blot analysis of Smad1 expression in 293T cell lysate.
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Immunohistochemical analysis of paraffin-embedded human colon, using Smad1 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For SMAD1 (Source: Uniprot.org, NCBI)
Gene Name
SMAD1
Full Name
Mothers against decapentaplegic homolog 1
Weight
52260 MW
Superfamily
dwarfin/SMAD family
Alternative Names
Mothers against decapentaplegic homolog 1;MAD homolog 1;Mothers against DPP homolog 1;JV4-1;Mad-related protein 1;SMAD family member 1;SMAD 1;Smad1;hSMAD1;Transforming growth factor-beta-signaling protein 1;BSP-1;SMAD1;BSP1, MADH1, MADR1; SMAD1 BSP-1, BSP1, JV4-1, JV41, MADH1, MADR1 SMAD family member 1 mothers against decapentaplegic homolog 1|MAD, mothers against decapentaplegic homolog 1|Mad-related protein 1|SMAD, mothers against DPP homolog 1|TGF-beta signaling protein 1|mothers against DPP homolog 1|transforming growth factor-beta signaling protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SMAD1, check out the SMAD1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SMAD1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Smad1 Rabbit Monoclonal Antibody (M00728)
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No publications found for M00728
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Customer Q&As
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16 Customer Q&As for Anti-Smad1 Rabbit Monoclonal Antibody
Question
I see that the anti-Smad1 Rabbit Monoclonal antibody M00728 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-10
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-03-10
Question
Is there a BSA free version of anti-Smad1 Rabbit Monoclonal antibody M00728 available?
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Smad1 Rabbit Monoclonal antibody M00728 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-24
Question
I was wanting to use your anti-Smad1 Rabbit Monoclonal antibody for IF for human kidney on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human kidney identification?
Verified Customer
Verified customer
Asked: 2019-12-02
Answer
It shows on the product datasheet, M00728 anti-Smad1 Rabbit Monoclonal antibody has been validated for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-02
Question
We have been able to see staining in human kidney. Are there any suggestions? Is anti-Smad1 Rabbit Monoclonal antibody supposed to stain kidney positively?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
According to literature kidney does express SMAD1. According to Uniprot.org, SMAD1 is expressed in visceral pleura, kidney, mammary carcinoma, placenta, uterus, ovarian carcinoma, among other tissues. Regarding which tissues have SMAD1 expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 8637600
Mammary carcinoma, Pubmed ID: 8663601
Ovarian carcinoma, Pubmed ID: 9136927
Placenta, Pubmed ID: 8774881, 15489334
Uterus, Pubmed ID: 14702039, 17974005
Boster Scientific Support
Answered: 2019-10-10
Question
Is a blocking peptide available for product anti-Smad1 Rabbit Monoclonal antibody (M00728)?
Verified Customer
Verified customer
Asked: 2019-09-10
Answer
We do provide the blocking peptide for product anti-Smad1 Rabbit Monoclonal antibody (M00728). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-10
Question
We have tried in the past anti-Smad1 Rabbit Monoclonal antibody for WB on uterus a few years ago. I am using human, and We are going to use the antibody for IF next. We are interested in examining uterus as well as visceral pleura in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
I looked at the website and datasheets of our anti-Smad1 Rabbit Monoclonal antibody and it appears that M00728 has been tested on human in both WB and IF. Thus M00728 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-07-26
Question
I am looking for using your anti-Smad1 Rabbit Monoclonal antibody for transforming growth factor beta receptor signaling pathway studies. Has this antibody been tested with western blotting on 293t cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-01-03
Answer
I appreciate your inquiry. This M00728 anti-Smad1 Rabbit Monoclonal antibody is tested on 293t cell lysate. It is guaranteed to work for IF, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-01-03
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney using anti-Smad1 Rabbit Monoclonal antibody M00728. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-12-10
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-10
Question
Does anti-Smad1 Rabbit Monoclonal antibody M00728 work for IF with kidney?
Verified Customer
Verified customer
Asked: 2018-08-20
Answer
According to the expression profile of kidney, SMAD1 is highly expressed in kidney. So, it is likely that anti-Smad1 Rabbit Monoclonal antibody M00728 will work for IF with kidney.
Boster Scientific Support
Answered: 2018-08-20
Question
I am interested in to test anti-Smad1 Rabbit Monoclonal antibody M00728 on human kidney for research purposes, then I may be interested in using anti-Smad1 Rabbit Monoclonal antibody M00728 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-03-08
Answer
The products we sell, including anti-Smad1 Rabbit Monoclonal antibody M00728, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-03-08
Question
I have a question about product M00728, anti-Smad1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-12-15
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00728 anti-Smad1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-12-15
Question
Here is the WB image, lot number and protocol we used for kidney using anti-Smad1 Rabbit Monoclonal antibody M00728. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-08-31
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-08-31
Question
Does M00728 anti-Smad1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Patel
Verified customer
Asked: 2016-04-15
Answer
You can see on the product datasheet, M00728 anti-Smad1 Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-04-15
Question
Is this M00728 anti-Smad1 Rabbit Monoclonal antibody reactive to the isotypes of SMAD1?
O. Anderson
Verified customer
Asked: 2015-05-07
Answer
The immunogen of M00728 anti-Smad1 Rabbit Monoclonal antibody is A synthesized peptide derived from human Smad1 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-05-07
Question
We were content with the WB result of your anti-Smad1 Rabbit Monoclonal antibody. However we have been able to see positive staining in uterus cytoplasm. using this antibody. Is that expected? Could you tell me where is SMAD1 supposed to be expressed?
D. Krishna
Verified customer
Asked: 2014-11-18
Answer
From literature, uterus does express SMAD1. Generally SMAD1 expresses in cytoplasm. Regarding which tissues have SMAD1 expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 8637600
Mammary carcinoma, Pubmed ID: 8663601
Ovarian carcinoma, Pubmed ID: 9136927
Placenta, Pubmed ID: 8774881, 15489334
Uterus, Pubmed ID: 14702039, 17974005
Boster Scientific Support
Answered: 2014-11-18
Question
We are currently using anti-Smad1 Rabbit Monoclonal antibody M00728 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?
L. Zhang
Verified customer
Asked: 2013-11-25
Answer
The anti-Smad1 Rabbit Monoclonal antibody (M00728) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-11-25
