Anti-Slc7a9 Antibody Picoband™

Product Name

Anti-Slc7a9 Antibody Picoband™

View all SLC7A9 Antibodies

SKU/Catalog Number

A03445-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Slc7a9 Antibody Picoband™ catalog # A03445-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Slc7a9 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03445-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived mouse Slc7a9 recombinant protein (Position: M1-E487).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A03445-1 is reactive to SLC7A9 in Mouse, Rat

Applications

A03445-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

53 kDa

Calculated molecular weight

31203 MW

Background of SLC7A9

b (0,+)-type amino acid transporter 1, also known as b (0,+)AT1, is a protein which in humans is encoded by the SLC7A9 gene. This gene encodes a protein that belongs to a family of light subunits of amino acid transporters. This protein plays a role in the high-affinity and sodium-independent transport of cystine and neutral and dibasic amino acids, and appears to function in the reabsorption of cystine in the kidney tubule. Mutations in this gene cause non-type I cystinuria, a disease that leads to cystine stones in the urinary system due to impaired transport of cystine and dibasic amino acids. Alternate transcript variants, which encode the same protein, have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Mouse
Direct ELISA, 0.1-0.5μg/ml, Mouse

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Slc7a9 using anti-Slc7a9 antibody (A03445-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat kidney tissue lysates,
Lane 2: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Slc7a9 antigen affinity purified polyclonal antibody (Catalog # A03445-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Slc7a9 at approximately 53 kDa. The expected band size for Slc7a9 is at 53 kDa.

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Figure 2. Flow Cytometry analysis of HEPA1-6 cells using anti-Slc7a9 antibody (A03445-1).
Overlay histogram showing HEPA1-6 cells stained with A03445-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Slc7a9 Antibody (A03445-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Gene/Protein Information For SLC7A9 (Source: Uniprot.org, NCBI)

Alternative Names

b(0,+)AT; b(0+)AT; B(0+)-type amino acid transporter 1; BAT1; bo+ amino acid transporter; CSNU3; FLJ94301; Glycoprotein-associated amino acid transporter b0+AT1; SLC7A9; solute carrier family 7 (cationic amino acid transporter, y+ system), member 9; Solute carrier family 7 member 9 SLC7A9 BAT1, CSNU3 solute carrier family 7 member 9 B(0,+)-type amino acid transporter 1|b(0,+)AT|glycoprotein-associated amino acid transporter b0,+AT1|solute carrier family 7 (amino acid transporter light chain, bo,+ system), member 9|solute carrier family 7 (cationic amino acid transporter, y+ system), member 9|solute carrier family 7 (glycoprotein-associated amino acid transporter light chain, bo,+ system), member 9

For more info on SLC7A9, check out the SLC7A9 Infographic

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In this infographic, you will see the following information for SLC7A9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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