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Product Info Summary
| SKU: | A03602-2 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-SLC7A7 Antibody Picoband®
SKU/Catalog Number
A03602-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-SLC7A7 Antibody Picoband® catalog # A03602-2. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SLC7A7 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03602-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human SLC7A7 recombinant protein (Position: M1-D498). Human SLC7A7 shares 90.8% and 91.6% amino acid (aa) sequence identity with mouse and rat SLC7A7, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03602-2 is reactive to SLC7A7 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
60 kDa
Calculated molecular weight
55.991kDa
Background of SLC7A7
Y+L amino acid transporter 1 is a protein that in humans is encoded by the SLC7A7 gene. The protein encoded by this gene is the light subunit of a cationic amino acid transporter. This sodium-independent transporter is formed when the light subunit encoded by this gene dimerizes with the heavy subunit transporter protein SLC3A2. This transporter is found in epithelial cell membranes where it transfers cationic and large neutral amino acids from the cell to the extracellular space. Defects in this gene are a cause of lysinuric protein intolerance (LPI). Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03602-2 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/ml, Human
Immunofluorescence, 5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Hela whole cell, human HepG2 whole cell, human 293T whole cell, rat heart tissue, rat brain tissue, mouse heart tissue, mouse brain tissue
IHC: human ovarian cancer tissue, human ovarian cancer tissue
IF: human ovarian cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SLC7A7 using anti-SLC7A7 antibody (A03602-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: rat heart tissue lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse heart tissue lysates,
Lane 7: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC7A7 antigen affinity purified polyclonal antibody (Catalog # A03602-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC7A7 at approximately 60 kDa. The expected band size for SLC7A7 is at 56 kDa.
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Figure 2. IHC analysis of SLC7A7 using anti-SLC7A7 antibody (A03602-2).
SLC7A7 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC7A7 Antibody (A03602-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 3. IHC analysis of SLC7A7 using anti-SLC7A7 antibody (A03602-2).
SLC7A7 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC7A7 Antibody (A03602-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. IF analysis of SLC7A7 using anti-SLC7A7 antibody (A03602-2).
SLC7A7 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SLC7A7 Antibody (A03602-2) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For SLC7A7 (Source: Uniprot.org, NCBI)
Gene Name
SLC7A7
Full Name
Y+L amino acid transporter 1
Weight
55.991kDa
Superfamily
amino acid-polyamine-organocation (APC) superfamily
Alternative Names
LAT3; LPI; Monocyte amino acid permease 2; MOP-2; SLC7A7; solute carrier family 7 (cationic amino acid transporter, y+ system), member 7; Solute carrier family 7 member 7; Y+L amino acid transporter 1; Y+LAT1; y+LAT-1y(+)L-type amino acid transporter 1 SLC7A7 LAT3, LPI, MOP-2, Y+LAT1, y+LAT-1 solute carrier family 7 member 7 Y+L amino acid transporter 1|monocyte amino acid permease 2|solute carrier family 7 (amino acid transporter light chain, y+L system), member 7|solute carrier family 7 (cationic amino acid transporter, y+ system), member 7|y(+)L-type amino acid transporter 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SLC7A7, check out the SLC7A7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SLC7A7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SLC7A7 Antibody Picoband® (A03602-2)
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