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Product Info Summary
| SKU: | PB9438 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Serotonin transporter/SLC6A4 Antibody Picoband®
View all SLC6A4/5-HTTLPR/Serotonin transporter Antibodies
SKU/Catalog Number
PB9438
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Serotonin transporter/SLC6A4 Antibody Picoband® catalog # PB9438. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Serotonin transporter/SLC6A4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9438)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human SLC6A4, different from the related mouse and rat sequences by six amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9438 is reactive to SLC6A4 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
90 kDa
Calculated molecular weight
70325 MW
Background of SLC6A4/5-HTTLPR/Serotonin transporter
SLC6A4 (Solute carrier family 6, member 4), also known as SERT (serotonin transporter), is a monoamine transporter protein. The SLC6A4 gene spans 31 kb and contains 14 exons. This protein integral membrane protein transports the neurotransmitter serotonin from synaptic spaces into presynaptic neurons. This transport of serotonin by the SERT protein terminates the action of serotonin and recycles it in a sodium-dependent manner. This protein is the target of many antidepressant medications, including those of the SSRI class. It is a member of the sodium: neurotransmitter symporter family. A repeat length polymorphism in the promoter of this gene has been shown to affect the rate of serotonin uptake and may play a role in sudden infant death syndrome, aggressive behavior in Alzheimer disease patients, post-traumatic stress disorder and depression-susceptibility in people experiencing emotional trauma.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9438 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat
Positive Control
WB: human HepG whole cell, human Caco-2 whole cell, human SW620 whole cell, human COLO-320 whole cellhuman K562 whole cell, human U2OS whole cell, human U-87MG whole cell, rat heart tissue, mouse heart tissue, mouse SP20 whole cell
IHC: rat brain tissue, human mammary cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SLC6A4 using anti-SLC6A4 antibody (PB9438).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HepG whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human SW620 whole cell lysates,
Lane 4: human COLO-320 whole cell lysates.
Lane 5: human K562 whole cell lysates,
Lane 6: human U2OS whole cell lysates,
Lane 7: human U-87MG whole cell lysates,
Lane 8: rat heart tissue lysates,
Lane 9: mouse heart tissue lysates,
Lane 10: mouse SP20 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC6A4 antigen affinity purified polyclonal antibody (Catalog # PB9438) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC6A4 at approximately 90KD. The expected band size for SLC6A4 is at 70KD.
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Figure 2. IHC analysis of SLC6A4 using anti-SLC6A4 antibody (PB9438). SLC6A4 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC6A4 Antibody (PB9438) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of SLC6A4 using anti-SLC6A4 antibody (PB9438). SLC6A4 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC6A4 Antibody (PB9438) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For SLC6A4 (Source: Uniprot.org, NCBI)
Gene Name
SLC6A4
Full Name
Sodium-dependent serotonin transporter
Weight
70325 MW
Superfamily
sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family
Alternative Names
Sodium-dependent serotonin transporter;5HT transporter;5HTT;Solute carrier family 6 member 4;SLC6A4;HTT, SERT; SLC6A4 5-HTT, 5-HTTLPR, 5HTT, HTT, OCD1, SERT, SERT1, hSERT solute carrier family 6 member 4 sodium-dependent serotonin transporter|5-hydroxytryptamine (serotonin) transporter|5HT transporter|Na+/Cl- dependent serotonin transporter|serotonin transporter 1|solute carrier family 6 (neurotransmitter transporter), member 4|solute carrier family 6 (neurotransmitter transporter, serotonin), member 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SLC6A4, check out the SLC6A4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SLC6A4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Serotonin transporter/SLC6A4 Antibody Picoband® (PB9438)
Hello CJ!
PB9438 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Liu ZJ,Liu H,Wu C,Xue K.Effect of sepsis on the action potential and cardiac serotonin response in rats.Exp Ther Med.2019 Sep;18(3):2207-2212.doi:10.3892/etm.2019.7810.Epub 2019 Jul 25.PMID:31452710;PMCID:PMC6704535.
Species: Rat
PB9438 usage in article: APP:IHC, SAMPLE:VENTRICULAR TISSUE, DILUTION:1:100
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Customer Q&As
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2 Customer Q&As for Anti-Serotonin transporter/SLC6A4 Antibody Picoband®
Question
We are currently using anti-Serotonin transporter/SLC6A4 antibody PB9438 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
The anti-Serotonin transporter/SLC6A4 antibody (PB9438) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-03
Question
For the Western Blots, were the Hela Cells transfected? Keywords: Hela Cell Transfection, Anti-SLC6A4 Antibody
Verified Customer
Verified customer
Asked: 2014-11-17
Answer
We do not transfect our cell lines for testings. The samples are not enriched/spiked.
Boster Scientific Support
Answered: 2014-11-17
