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Product Info Summary
| SKU: | A10812-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-SLC6A18 Antibody Picoband®
SKU/Catalog Number
A10812-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SLC6A18 Antibody Picoband® catalog # A10812-1. Tested in ELISA, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SLC6A18 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10812-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SLC6A18 recombinant protein (Position: M1-A569).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A10812-1 is reactive to SLC6A18 in Human, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
71 kDa
Calculated molecular weight
29370 MW
Background of Slc6a18
Solute carrier family 6, member 18 also known as SLC6A18 is a protein which in humans is encoded by the SLC6A18 gene. The SLC6 family of proteins, which includes SLC6A18, act as specific transporters for neurotransmitters, amino acids, and osmolytes like betaine, taurine, and creatine. SLC6 proteins are sodium cotransporters that derive the energy for solute transport from the electrochemical gradient for sodium ions.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10812-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human, Rat
ELISA, 0.1-0.5 µg/ml, Human
Positive Control
WB: human 293T whole cell, rat kidney tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SLC6A18 using anti-SLC6A18 antibody (A10812-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde
r reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: rat kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC6A18 antigen affinity purified polyclonal antibody (Catalog # A10812-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC6A18 at approximately 71 kDa. The expected band size for SLC6A18 is at 71 kDa.
Protein Target Info & Infographic
Gene/Protein Information For SLC6A18 (Source: Uniprot.org, NCBI)
Gene Name
SLC6A18
Full Name
Sodium-dependent neutral amino acid transporter B(0)AT3
Weight
29370 MW
Superfamily
sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family
Alternative Names
RNA-binding protein Nova-2; Astrocytic NOVA1-like RNA-binding protein; Neuro-oncological ventral antigen 2; NOVA2; ANOVA; NOVA3 Slc6a18|B0AT3, D630001K16Rik, Xt2, Xtr, Xtrp2|solute carrier family 6 (neurotransmitter transporter), member 18|sodium-dependent neutral amino acid transporter B(0)AT3|X transporter protein 2|b(0)AT3|sodium and chloride dependent transporter Xtrp2|sodium- and chloride-dependent transporter XTRP2|solute carrier family 6 member 18|system B 0 neutral amino acid transporter AT3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SLC6A18, check out the SLC6A18 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SLC6A18: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SLC6A18 Antibody Picoband® (A10812-1)
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