Anti-SLC5A7 Antibody Picoband™

Product Name

Anti-SLC5A7 Antibody Picoband™

View all CHT1 Antibodies

SKU/Catalog Number

A05277-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SLC5A7 Antibody Picoband™ catalog # A05277-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-SLC5A7 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05277-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human SLC5A7 recombinant protein (Position: R446-Q580).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A05277-1 is reactive to SLC5A7 in Human, Mouse, Rat

Applications

A05277-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

80 kDa

Calculated molecular weight

39411 MW

Background of CHT1

The high-affinity choline transporter (ChT) also known as solute carrier family 5 member 7 is a protein in humans that is encoded by the SLC5A7 gene. This gene encodes a sodium ion- and chloride ion-dependent high-affinity transporter that mediates choline uptake for acetylcholine synthesis in cholinergic neurons. The protein transports choline from the extracellular space into presynaptic terminals for synthesis into acetylcholine. Increased choline uptake results from increased density of this protein in synaptosomal plasma membranes in response to depolarization of cholinergic terminals. Dysfunction of cholinergic signaling has been implicated in various disorders including depression, attention-deficit disorder, and schizophrenia. An allelic variant of this gene is associated with autosomal dominant distal hereditary motor neuronopathy type VIIA. Alternative splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/ml/1x10^6 cells, Human
Direct ELISA, 0.1-0.5 μg/ml/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of SLC5A7 using anti-SLC5A7 antibody (A05277-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: humna RT4 whle cell lysates,
Lane 3: rat C6 whole cell lysates,
Lane 4: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC5A7 antigen affinity purified polyclonal antibody (Catalog # A05277-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC5A7 at approximately 80 kDa. The expected band size for SLC5A7 is at 63 kDa.

Click image to see more details

Figure 2. Flow Cytometry analysis of JK cells using anti-SLC5A7 antibody (A05277-1).
Overlay histogram showing JK cells stained with A05277-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC5A7 Antibody (A05277-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Gene/Protein Information For SLC5A7 (Source: Uniprot.org, NCBI)

Alternative Names

CHT; CHT1MGC126299; hCHT; Hemicholinium-3-sensitive choline transporter; high affinity choline transporter 1; high affinity choline transporter; hemicholinium-3-sensitive cholinetransporter; MGC126300; solute carrier family 5 (choline transporter), member 7; Solute carrier family 5 member 7 SLC5A7 CHT, CHT1, CMS20, HMN7A solute carrier family 5 member 7 high affinity choline transporter 1|high affinity choline transporter; hemicholinium-3-sensitive choline transporter|solute carrier family 5 (sodium/choline cotransporter), member 7

For more info on SLC5A7, check out the SLC5A7 Infographic

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In this infographic, you will see the following information for SLC5A7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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