Click image to see more details
Product Info Summary
| SKU: | PB9436 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Glucose Transporter GLUT2/SLC2A2 Antibody Picoband®
SKU/Catalog Number
PB9436
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Glucose Transporter GLUT2/SLC2A2 Antibody Picoband® catalog # PB9436. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Glucose Transporter GLUT2/SLC2A2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9436)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human SLC2A2, different from the related mouse sequence by five amino acids, and from the related rat sequence by seven amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9436 is reactive to SLC2A2 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
50 kDa
Calculated molecular weight
57490 MW
Background of Glut2
SLC2A2, also known as Glucose transporter 2 (GLUT2), is a transmembrane carrier protein that enables protein facilitatedglucose movement across cell membranes. This gene encodes an integral plasma membrane glycoprotein of the liver, islet beta cells, intestine, and kidney epithelium. The encoded protein mediates facilitated bidirectional glucose transport. Because of its low affinity for glucose, it has been suggested as a glucose sensor. Mutations in this gene are associated with susceptibility to diseases, including Fanconi-Bickel syndrome and noninsulin-dependent diabetes mellitus (NIDDM). Alternative splicing results in multiple transcript variants of this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9436 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: PANC Whole Cell, A549 Whole Cell, HT1080 Whole Cell
IHC: human liver cancer tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of SLC2A2 using anti-SLC2A2 antibody (PB9436).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: PANC Whole Cell Lysate at 40ug,
Lane 2: A549 Whole Cell Lysate at 40ug,
Lane 3: HT1080 Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC2A2 antigen affinity purified polyclonal antibody (Catalog # PB9436) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC2A2 at approximately 50 kDa. The expected band size for SLC2A2 is at 57 kDa.
Click image to see more details
Figure 2. IHC analysis of SLC2A2 using anti-SLC2A2 antibody (PB9436).
SLC2A2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SLC2A2 Antibody (PB9436) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For SLC2A2 (Source: Uniprot.org, NCBI)
Gene Name
SLC2A2
Full Name
Solute carrier family 2, facilitated glucose transporter member 2
Weight
57490 MW
Superfamily
major facilitator superfamily
Alternative Names
Solute carrier family 2, facilitated glucose transporter member 2;Glucose transporter type 2, liver;GLUT-2;SLC2A2;GLUT2; SLC2A2 GLUT2 solute carrier family 2 member 2 solute carrier family 2, facilitated glucose transporter member 2|glucose transporter type 2, liver|solute carrier family 2 (facilitated glucose transporter), member 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SLC2A2, check out the SLC2A2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SLC2A2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Glucose Transporter GLUT2/SLC2A2 Antibody Picoband® (PB9436)
Hello CJ!
PB9436 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Indomethacin decreases insulin secretion by reducing KCa3.1 as a biomarker of pancreatic tumor and causes apoptotic cell death
Impact of perinatal exposure to acetaminophen on hepatocellular metabolic function in offspring
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Glucose Transporter GLUT2/SLC2A2 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Glucose Transporter GLUT2/SLC2A2 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
1 Customer Q&As for Anti-Glucose Transporter GLUT2/SLC2A2 Antibody Picoband®
Question
We are currently using anti-Glucose Transporter GLUT2/SLC2A2 antibody PB9436 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on pig tissues as well?
J. Edwards
Verified customer
Asked: 2019-07-26
Answer
The anti-Glucose Transporter GLUT2/SLC2A2 antibody (PB9436) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-26
