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Product Info Summary
| SKU: | M00163 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-GLUT1 SLC2A1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00163
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-GLUT1 SLC2A1 Rabbit Monoclonal Antibody catalog # M00163. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GLUT1 SLC2A1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00163)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
CGG-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Glucose Transporter GLUT1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00163 is reactive to SLC2A1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
72 kDa
Calculated molecular weight
54084 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00163 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Positive Control
WB: HepG2
IHC: Human stomach tissue, Rat hippocampus tissue, Mouse kidney tissue, Human thymoma tissue, Human colon tissue, human cervix cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of GLUT1 expression in HepG2 lysate.
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Immunohistochemical analysis of paraffin-embedded Human stomach, using the Antibody at 1:800 dilution.
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Immunohistochemical analysis of paraffin-embedded Rat hippocampus , using the Antibody at 1:300 dilution.
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Immunohistochemical analysis of paraffin-embedded Mouse kidney, using the Antibody at 1:300 dilution.
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Immunohistochemical analysis of paraffin-embedded Human thymoma, using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded Human colon, using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded human cervix cancer, using GLUT1 Antibody.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis of HepG2 cells, using GLUT1 Antibody .
Protein Target Info & Infographic
Gene/Protein Information For SLC2A1 (Source: Uniprot.org, NCBI)
Gene Name
SLC2A1
Full Name
Solute carrier family 2, facilitated glucose transporter member 1
Weight
54084 MW
Superfamily
major facilitator superfamily
Alternative Names
Solute carrier family 2, facilitated glucose transporter member 1;Glucose transporter type 1, erythrocyte/brain;GLUT-1;HepG2 glucose transporter;SLC2A1;GLUT1; SLC2A1 CSE, DYT17, DYT18, DYT9, EIG12, GLUT, GLUT-1, GLUT1, GLUT1DS, HTLVR, PED, SDCHCN solute carrier family 2 member 1 solute carrier family 2, facilitated glucose transporter member 1|choreoathetosis/spasticity, episodic (paroxysmal choreoathetosis/spasticity)|glucose transporter type 1, erythrocyte/brain|hepG2 glucose transporter|human T-cell leukemia virus (I and II) receptor|receptor for HTLV-1 and HTLV-2|solute carrier family 2 (facilitated glucose transporter), member 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SLC2A1, check out the SLC2A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SLC2A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GLUT1 SLC2A1 Rabbit Monoclonal Antibody (M00163)
Hello CJ!
M00163 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Chen J,Wu D,Dong Z,Chen A,Liu S.The expression and role of glycolysis-associated molecules in infantile hemangioma.Life Sci.2020 Oct 15;259:118215.doi:10.1016/j.lfs.2020.118215.Epub 2020 Aug 5.PMID:32768579.
Species: Human
M00163 usage in article: APP:IHC, SAMPLE:NORMAL AND TUMOR SKIN TISSUE, DILUTION:1:200
Co-Inhibition of GLUT-1 Expression and the PI3K/Akt Signaling Pathway to Enhance the Radiosensitivity of Laryngeal Carcinoma Xenografts?In Vivo
Apigenin inhibits the proliferation of adenoid cystic carcinoma via suppression of glucose transporter-1
Xu Yy, Wu Tt, Zhou Sh, Bao Yy, Wang Qy, Fan J, Huang Yp. Int J Clin Exp Pathol. 2014 Jun 15;7(7):3938-47. Ecollection 2014. Apigenin Suppresses Glut-1 And P-Akt Expression To Enhance The Chemosensitivity To Cisplatin Of Laryngeal Carcinoma Hep-2 C...
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Customer Reviews
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Customer Q&As
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17 Customer Q&As for Anti-GLUT1 SLC2A1 Rabbit Monoclonal Antibody
Question
We ordered your anti-GLUT1 Rabbit Monoclonal antibody for WB on brain trachea a few months ago. I am using human, and We want to use the antibody for ICC next. I was wanting to use examining brain trachea as well as melanoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for ICC?
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
I looked at the website and datasheets of our anti-GLUT1 Rabbit Monoclonal antibody and it appears that M00163 has been validated on human in both WB and ICC. Thus M00163 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-03-23
Question
My question regarding product M00163, anti-GLUT1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00163 anti-GLUT1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-13
Question
Does anti-GLUT1 Rabbit Monoclonal antibody M00163 work on primate IHC with pigmented layer of retina?
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
Our lab technicians have not validated anti-GLUT1 Rabbit Monoclonal antibody M00163 on primate. You can run a BLAST between primate and the immunogen sequence of anti-GLUT1 Rabbit Monoclonal antibody M00163 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate pigmented layer of retina in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-29
Question
We are currently using anti-GLUT1 Rabbit Monoclonal antibody M00163 for human tissue, and we are content with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
The anti-GLUT1 Rabbit Monoclonal antibody (M00163) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-03
Question
Would M00163 anti-GLUT1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-11
Answer
It shows on the product datasheet, M00163 anti-GLUT1 Rabbit Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-11
Question
Would anti-GLUT1 Rabbit Monoclonal antibody M00163 work for IHC with pigmented layer of retina?
Verified Customer
Verified customer
Asked: 2019-09-16
Answer
According to the expression profile of pigmented layer of retina, SLC2A1 is highly expressed in pigmented layer of retina. So, it is likely that anti-GLUT1 Rabbit Monoclonal antibody M00163 will work for IHC with pigmented layer of retina.
Boster Scientific Support
Answered: 2019-09-16
Question
Is this M00163 anti-GLUT1 Rabbit Monoclonal antibody reactive to the isotypes of SLC2A1?
S. Mangal
Verified customer
Asked: 2019-09-09
Answer
The immunogen of M00163 anti-GLUT1 Rabbit Monoclonal antibody is A synthesized peptide derived from human Glucose Transporter GLUT1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-09
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pigmented layer of retina using anti-GLUT1 Rabbit Monoclonal antibody M00163. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-17
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-17
Question
Do you have a BSA free version of anti-GLUT1 Rabbit Monoclonal antibody M00163 available?
Verified Customer
Verified customer
Asked: 2019-02-08
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-GLUT1 Rabbit Monoclonal antibody M00163 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-02-08
Question
I see that the anti-GLUT1 Rabbit Monoclonal antibody M00163 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-01-10
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-01-10
Question
We have seen staining in human brain. Any tips? Is anti-GLUT1 Rabbit Monoclonal antibody supposed to stain brain positively?
S. Brown
Verified customer
Asked: 2018-09-24
Answer
According to literature brain does express SLC2A1. According to Uniprot.org, SLC2A1 is expressed in pigmented layer of retina, brain trachea, brain, articular cartilage, melanoma, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have SLC2A1 expression, here are a few articles citing expression in various tissues:
Brain, and Trachea, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19349973
Liver, Pubmed ID: 24275569
Melanoma, Pubmed ID: 17081065
Boster Scientific Support
Answered: 2018-09-24
Question
I would like to test anti-GLUT1 Rabbit Monoclonal antibody M00163 on human pigmented layer of retina for research purposes, then I may be interested in using anti-GLUT1 Rabbit Monoclonal antibody M00163 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
G. Walker
Verified customer
Asked: 2018-06-19
Answer
The products we sell, including anti-GLUT1 Rabbit Monoclonal antibody M00163, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-06-19
Question
My lab would like using your anti-GLUT1 Rabbit Monoclonal antibody for response to insulin studies. Has this antibody been tested with western blotting on hepg2 cells? We would like to see some validation images before ordering.
L. Jones
Verified customer
Asked: 2016-11-17
Answer
I appreciate your inquiry. This M00163 anti-GLUT1 Rabbit Monoclonal antibody is validated on hepg2 cells, hela cells. It is guaranteed to work for Flow Cytometry, IF, IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-11-17
Question
See below the WB image, lot number and protocol we used for pigmented layer of retina using anti-GLUT1 Rabbit Monoclonal antibody M00163. Please let me know if you require anything else.
J. Krishna
Verified customer
Asked: 2014-08-14
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-08-14
Question
Is a blocking peptide available for product anti-GLUT1 Rabbit Monoclonal antibody (M00163)?
J. Miller
Verified customer
Asked: 2014-05-07
Answer
We do provide the blocking peptide for product anti-GLUT1 Rabbit Monoclonal antibody (M00163). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-05-07
Question
I was wanting to use your anti-GLUT1 Rabbit Monoclonal antibody for IHC for human pigmented layer of retina on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human pigmented layer of retina identification?
J. Patel
Verified customer
Asked: 2013-11-11
Answer
As indicated on the product datasheet, M00163 anti-GLUT1 Rabbit Monoclonal antibody has been validated for Flow Cytometry, IF, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human pigmented layer of retina in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-11-11
Question
My team were well pleased with the WB result of your anti-GLUT1 Rabbit Monoclonal antibody. However we have observed positive staining in cervix carcinoma erythroleukemia cell membrane using this antibody. Is that expected? Could you tell me where is SLC2A1 supposed to be expressed?
A. Jones
Verified customer
Asked: 2013-05-30
Answer
From what I have seen in literature, cervix carcinoma erythroleukemia does express SLC2A1. Generally SLC2A1 expresses in cell membrane. Regarding which tissues have SLC2A1 expression, here are a few articles citing expression in various tissues:
Brain, and Trachea, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19349973
Liver, Pubmed ID: 24275569
Melanoma, Pubmed ID: 17081065
Boster Scientific Support
Answered: 2013-05-30
