Product Info Summary
SKU: | A08997-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-SLC25A23 Antibody Picoband™
SKU/Catalog Number
A08997-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SLC25A23 Antibody Picoband™ catalog # A08997-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SLC25A23 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08997-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SLC25A23 recombinant protein (Position: R16-L189).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A08997-1 is reactive to Slc25a23 in Human, Mouse, Rat
Applications
A08997-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
48-54 kDa
Calculated molecular weight
Background of Slc25a23
Predicted to enable ATP transmembrane transporter activity. Involved in calcium import into the mitochondrion; positive regulation of mitochondrial calcium ion concentration; and regulation of cellular hyperosmotic salinity response. Located in mitochondrion.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 µg/ml, Mouse, Rat
Direct ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
![a08997 1 slc25a3 primary antibodies wb testing 1 a08997 1 slc25a3 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a08997-1-slc25a3-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of SLC25A23 using anti-SLC25A23 antibody (A08997-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tisue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC25A23 antigen affinity purified polyclonal antibody (Catalog # A08997-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC25A23 at approximately 48-54 kDa. The expected band size for SLC25A23 is at 52 kDa.
![a08997 1 slc25a3 primary antibodies ihc testing 2 a08997 1 slc25a3 primary antibodies ihc testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a08997-1-slc25a3-primary-antibodies-ihc-testing-2.jpg)
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Figure 2. IHC analysis of SLC25A23 using anti-SLC25A23 antibody (A08997-1).
SLC25A23 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC25A23 Antibody (A08997-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a08997 1 slc25a3 primary antibodies ihc testing 3 a08997 1 slc25a3 primary antibodies ihc testing 3](https://www.bosterbio.com/media/catalog/product/a/0/a08997-1-slc25a3-primary-antibodies-ihc-testing-3.jpg)
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Figure 3. IHC analysis of SLC25A23 using anti-SLC25A23 antibody (A08997-1).
SLC25A23 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC25A23 Antibody (A08997-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Slc25a23 (Source: Uniprot.org, NCBI)
Gene Name
Slc25a23
Full Name
Calcium-binding mitochondrial carrier protein SCaMC-3
Weight
Superfamily
mitochondrial carrier (TC 2.A.29) family
Alternative Names
APC2FLJ30339; calcium-binding mitochondrial carrier protein SCaMC-3; MCSC2; MGC2615; Mitochondrial ATP-Mg/Pi carrier protein 2; Mitochondrial Ca(2+)-dependent solute carrier protein 2; mitochondrial Ca2+-dependent solute carrier protein 2; SCAMC3; SCaMC-3; short calcium-binding mitochondrial carrier 3; Small calcium-binding mitochondrial carrier protein 3; solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 23; Solute carrier family 25 member 23
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Slc25a23, check out the Slc25a23 Infographic
![Slc25a23 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Slc25a23: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SLC25A23 Antibody Picoband™ (A08997-1)
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