Anti-SLC22A6 Antibody

Product Name

Anti-SLC22A6 Antibody

View all SLC22A6 Antibodies

SKU/Catalog Number

PA1683

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SLC22A6 Antibody catalog # PA1683. Tested in IHC, WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SLC22A6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1683)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human SLC22A6, different from the related rat sequence by three amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1683 is reactive to SLC22A6 in Human, Rat

Applications

PA1683 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

36 kDa

Calculated molecular weight

61816 MW

Background of SLC22A6

SLC22A6 (Solute carrier family 22 (organic anion transporter), member 6), also called OAT1 or PAHT, is a protein that in humans is encoded by the SLC22A6 gene, which is also a member of the organic anion transporter (OAT) family of proteins. OAT1 is a transmembrane protein that is expressed in the brain, the placenta, the eyes, smooth muscles, and the basolateral membrane of proximal tubular cells of the kidneys. The SLC22A6 gene is mapped on 11q12.3. It plays a central role in renal organic anion transport. Along with OAT3, OAT1 mediates the uptake of a wide range of relatively small and hydrophilic organic anions from plasma into the cytoplasm of the proximal tubular cells of the kidneys. The SLC22A6 gene contains 10 exons and spans 8.2 kb. OAT1 functions as organic anion exchanger. When the uptake of one molecule of an organic anion is transported into a cell by an OAT1 exchanger, one molecule of an endogenous dicarboxylic acid (such as glutarate, ketoglutarate, etc) is simultaneously transported out of the cell. PAH uptake in Xenopus oocytes injected with OAT1 mRNA was demonstrated by Race et al.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

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Anti-SLC22A6 antibody, PA1683, IHC(P)
IHC(P): Rat Kidney Tissue

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Anti-SLC22A6 antibody, PA1683, Western blotting
Lane 1: HT1080 Cell Lysate
Lane 2: HELA Cell Lysate

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Figure 3. IHC analysis of SLC22A6 using anti-SLC22A6 antibody (PA1683).
SLC22A6 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC22A6 Antibody (PA1683) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Gene/Protein Information For SLC22A6 (Source: Uniprot.org, NCBI)

Alternative Names

hOAT1; hPAHT; hROAT1; MGC45260; OAT1; OAT1FLJ55736; Organic anion transporter 1; PAH transporter; PAHT; PAHTHOAT1; para-aminohippurate transporter; Renal organic anion transporter 1; ROAT1; SLC22A6; solute carrier family 22 (organic anion transporter), member 6; solute carrier family 22 member 6 SLC22A6 HOAT1, OAT1, PAHT, ROAT1 solute carrier family 22 member 6 solute carrier family 22 member 6|PAH transporter|hPAHT|hROAT1|organic anion transporter 1|para-aminohippurate transporter|renal organic anion transporter 1|solute carrier family 22 (organic anion transporter), member 6

For more info on SLC22A6, check out the SLC22A6 Infographic

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In this infographic, you will see the following information for SLC22A6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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