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Product Info Summary
| SKU: | PA1683 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-SLC22A6 Antibody Picoband®
SKU/Catalog Number
PA1683
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SLC22A6 Antibody catalog # PA1683. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SLC22A6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1683)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human SLC22A6, different from the related rat sequence by three amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1683 is reactive to SLC22A6 in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
36 kDa
Calculated molecular weight
61816 MW
Background of SLC22A6
SLC22A6 (Solute carrier family 22 (organic anion transporter), member 6), also called OAT1 or PAHT, is a protein that in humans is encoded by the SLC22A6 gene, which is also a member of the organic anion transporter (OAT) family of proteins. OAT1 is a transmembrane protein that is expressed in the brain, the placenta, the eyes, smooth muscles, and the basolateral membrane of proximal tubular cells of the kidneys. The SLC22A6 gene is mapped on 11q12.3. It plays a central role in renal organic anion transport. Along with OAT3, OAT1 mediates the uptake of a wide range of relatively small and hydrophilic organic anions from plasma into the cytoplasm of the proximal tubular cells of the kidneys. The SLC22A6 gene contains 10 exons and spans 8.2 kb. OAT1 functions as organic anion exchanger. When the uptake of one molecule of an organic anion is transported into a cell by an OAT1 exchanger, one molecule of an endogenous dicarboxylic acid (such as glutarate, ketoglutarate, etc) is simultaneously transported out of the cell. PAH uptake in Xenopus oocytes injected with OAT1 mRNA was demonstrated by Race et al.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1683 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: HT1080 Cell, HELA Cell,
IHC: Rat Kidney Tissue, human mammary cancer tissue
Validation Images & Assay Conditions
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Anti-SLC22A6 antibody, PA1683, IHC(P)
IHC(P): Rat Kidney Tissue
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Anti-SLC22A6 antibody, PA1683, Western blotting
Lane 1: HT1080 Cell Lysate
Lane 2: HELA Cell Lysate
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Figure 3. IHC analysis of SLC22A6 using anti-SLC22A6 antibody (PA1683).
SLC22A6 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC22A6 Antibody (PA1683) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For SLC22A6 (Source: Uniprot.org, NCBI)
Gene Name
SLC22A6
Full Name
Solute carrier family 22 member 6
Weight
61816 MW
Superfamily
major facilitator (TC 2.A.1) superfamily
Alternative Names
Solute carrier family 22 member 6;Organic anion transporter 1;hOAT1;PAH transporter;hPAHT;Renal organic anion transporter 1;hROAT1;SLC22A6;OAT1, PAHT; SLC22A6 HOAT1, OAT1, PAHT, ROAT1 solute carrier family 22 member 6 solute carrier family 22 member 6|PAH transporter|hPAHT|hROAT1|organic anion transporter 1|para-aminohippurate transporter|renal organic anion transporter 1|solute carrier family 22 (organic anion transporter), member 6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SLC22A6, check out the SLC22A6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SLC22A6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SLC22A6 Antibody Picoband® (PA1683)
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Customer Q&As
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5 Customer Q&As for Anti-SLC22A6 Antibody Picoband®
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for colon using anti-SLC22A6 antibody PA1683. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-13
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-13
Question
Do you have a BSA free version of anti-SLC22A6 antibody PA1683 available?
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-SLC22A6 antibody PA1683 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-12
Question
We are currently using anti-SLC22A6 antibody PA1683 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
The anti-SLC22A6 antibody (PA1683) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-31
Question
Will anti-SLC22A6 antibody PA1683 work on zebrafish IHC-P with colon?
Verified Customer
Verified customer
Asked: 2019-02-08
Answer
Our lab technicians have not validated anti-SLC22A6 antibody PA1683 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-SLC22A6 antibody PA1683 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish colon in IHC-P, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-02-08
Question
Does PA1683 anti-SLC22A6 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
K. Zhang
Verified customer
Asked: 2018-01-10
Answer
You can see on the product datasheet, PA1683 anti-SLC22A6 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-01-10
