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Product Info Summary
| SKU: | A09067 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-SLC1A7 Antibody
SKU/Catalog Number
A09067
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SLC1A7 Antibody (Catalog # A09067). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
SLC1A7 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-SLC1A7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09067)
Host
Rabbit
Contents
SLC1A7 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
SLC1A7 antibody was raised against a 14 amino acid peptide near the carboxy terminus of human SLC1A7. The immunogen is located within amino acids 460 - 510 of SLC1A7.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
SLC1A7 antibody is human and mouse reactive. Multiple isoforms of SLC1A7 are known to exist.
Reactive Species
A09067 is reactive to SLC1A7 in Human, Mouse
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
60658 MW
Background of SLC1A7
The solute carrier family 1 member 7 (SLC1A7) protein was initially identified as a glutamate transporter coupled to a chloride conductance that is expressed primarily in the retina (1). More recent evidence indicates that is it widely expressed in peripheral tissues, also (2). Experiments have shown that SLC1A7 functions as a low-affinity/low-capacity glutamate transport, with an anion channel optimized for anion conductance in the negative voltage range (3). In the retina, SLC1A7 co-localizes with the serum and glucocorticoid dependent kinase SGK1, and its cell surface expression and activity is regulated SGK1 and its isoform SGK3 (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09067 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SLC1A7 antibody can be used for detection of SLC1A7 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SLC1A7 in C2C12 cell lysate with SLC1A7 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of SLC1A7 in mouse kidney tissue with SLC1A7 antibody at 5 μg/ml.
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Immunofluorescence of SLC1A7 in mouse kidney tissue with SLC1A7 antibody at 20 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For SLC1A7 (Source: Uniprot.org, NCBI)
Gene Name
SLC1A7
Full Name
Excitatory amino acid transporter 5
Weight
60658 MW
Superfamily
dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family
Alternative Names
Solute carrier family 1 (glutamate transporter) member 7, AAAT, excitatory amino acid transporter 5, EAAT5 SLC1A7 AAAT, EAAT5 solute carrier family 1 member 7 excitatory amino acid transporter 5|excitatory amino acid transporter 5 (retinal glutamate transporter)|retinal glutamate transporter|solute carrier family 1 (glutamate transporter), member 7
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SLC1A7, check out the SLC1A7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SLC1A7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SLC1A7 Antibody (A09067)
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