Click image to see more details
Product Info Summary
| SKU: | A04926-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-SLC14A1/UTE Antibody Picoband®
SKU/Catalog Number
A04926-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SLC14A1/UTE Antibody Picoband® catalog # A04926-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SLC14A1/UTE Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04926-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human SCEL, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04926-1 is reactive to Slc14a1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
48-50 kDa
Calculated molecular weight
56903 MW
Background of SLC14A1
Urea transporter 1 is a protein that in humans is encoded by the SLC14A1 gene. The SLC14A1 codes for a urea transporter (UTB) that is expressed in erythrocytes and kidney. SLC14A2 and SLC14A1 constitute solute carrier family 14. UTB proteins constitute the Kidd antigen system.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04926-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Mouse
Positive Control
WB: mouse kidney tissue, mouse skeletal muscle tissue, mouse heart tissue, rat heart tissue, rat kidney tissue
FCM: HEPA1-6 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of SLC14A1/UTE using anti-SLC14A1/UTE antibody (A04926-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse kidney tissue lysates,
Lane 2: mouse skeletal muscle tissue lysates,
Lane 3: mouse heart tissue lysates,
Lane 4: rat heart tissue lysates,
Lane 5: rat kidney tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC14A1/UTE antigen affinity purified polyclonal antibody (Catalog # A04926-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC14A1/UTE at approximately 48-50KD. The expected band size for SLC14A1/UTE is at 42KD.
Click image to see more details
Figure 2. Flow Cytometry analysis of HEPA1-6 cells using anti-SLC14A1/UTE antibody (A04926-1).
Overlay histogram showing HEPA1-6 cells stained with A04926-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC14A1/UTE Antibody (A04926-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For Slc14a1 (Source: Uniprot.org, NCBI)
Gene Name
Slc14a1
Full Name
Urea transporter 1
Weight
56903 MW
Superfamily
urea transporter family
Alternative Names
Meiotic recombination protein REC8 homolog; Cohesin Rec8p; REC8; REC8L1 SLC14A1 HUT11, HsT1341, JK, Jk(b), RACH1, RACH2, UT-B1, UT1, UTE solute carrier family 14 member 1 (Kidd blood group) urea transporter 1|Kidd blood group |SLC14A1 JK|solute carrier family 14 (urea transporter), member 1 (Kidd blood group)|urea transporter JK glycoprotein|urea transporter, erythrocyte|urea transporter-B1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Slc14a1, check out the Slc14a1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Slc14a1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SLC14A1/UTE Antibody Picoband® (A04926-1)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-SLC14A1/UTE Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-SLC14A1/UTE Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
