Anti-SLC14A1/UTE Antibody Picoband™

Product Name

Anti-SLC14A1/UTE Antibody Picoband™

View all SLC14A1 Antibodies

SKU/Catalog Number

A04926-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SLC14A1/UTE Antibody Picoband™ catalog # A04926-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SLC14A1/UTE Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04926-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human SCEL, identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A04926-1 is reactive to SLC14A1 in Human, Mouse, Rat

Applications

A04926-1 is guaranteed for Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

48-50 kDa

Calculated molecular weight

56903 MW

Background of SLC14A1

Urea transporter 1 is a protein that in humans is encoded by the SLC14A1 gene. The SLC14A1 codes for a urea transporter (UTB) that is expressed in erythrocytes and kidney. SLC14A2 and SLC14A1 constitute solute carrier family 14. UTB proteins constitute the Kidd antigen system.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Mouse, Rat
Flow Cytometry, 1-3μg/1x10⁶ cells, Mouse

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of SLC14A1/UTE using anti-SLC14A1/UTE antibody (A04926-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse kidney tissue lysates,
Lane 2: mouse skeletal muscle tissue lysates,
Lane 3: mouse heart tissue lysates,
Lane 4: rat heart tissue lysates,
Lane 5: rat kidney tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC14A1/UTE antigen affinity purified polyclonal antibody (Catalog # A04926-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC14A1/UTE at approximately 48-50KD. The expected band size for SLC14A1/UTE is at 42KD.

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Figure 2. Flow Cytometry analysis of HEPA1-6 cells using anti-SLC14A1/UTE antibody (A04926-1).
Overlay histogram showing HEPA1-6 cells stained with A04926-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC14A1/UTE Antibody (A04926-1, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Gene/Protein Information For SLC14A1 (Source: Uniprot.org, NCBI)

Alternative Names

FLJ33745; HsT1341; HUT11; JK; JKFLJ41687; RACH1; RACH2; SLC14A1; solute carrier family 14 (urea transporter), member 1 (Kidd blood group); Solute carrier family 14 member 1; truncated urea transporter; urea transporter 1; urea transporter JK glycoprotein; Urea transporter, erythrocyte; urea transporter-B1; UT1; UT1UT-B1; UT-B1; UTE; UTEblood group Kidd urea transporter SLC14A1 HUT11, HsT1341, JK, Jk(b), RACH1, RACH2, UT-B1, UT1, UTE solute carrier family 14 member 1 (Kidd blood group) urea transporter 1|Kidd blood group antigen|SLC14A1 JK|solute carrier family 14 (urea transporter), member 1 (Kidd blood group)|urea transporter JK glycoprotein|urea transporter, erythrocyte|urea transporter-B1

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