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Product Info Summary
| SKU: | A06502-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Homeobox protein SIX6 Antibody Picoband®
SKU/Catalog Number
A06502-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Homeobox protein SIX6 Antibody Picoband® catalog # A06502-1. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Homeobox protein SIX6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06502-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human SIX6 recombinant protein (Position: A188-I246). Human SIX6 shares 91.5% amino acid (aa) sequence identity with mouse SIX6.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A06502-1 is reactive to SIX6 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
28 kDa
Calculated molecular weight
27687 MW
Background of SIX6
The protein encoded by this gene is a homeobox protein that is similar to the Drosophila 'sine oculis' gene product. This gene is found in a cluster of related genes on chromosome 14 and is thought to be involved in eye development. Defects in this gene are a cause of isolated microphthalmia with cataract type 2 (MCOPCT2).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06502-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human Hela whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of SIX6 using anti-SIX6 antibody (A06502-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIX6 antigen affinity purified polyclonal antibody (Catalog # A06502-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIX6 at approximately 28KD. The expected band size for SIX6 is at 28KD.
Protein Target Info & Infographic
Gene/Protein Information For SIX6 (Source: Uniprot.org, NCBI)
Gene Name
SIX6
Full Name
Homeobox protein SIX6
Weight
27687 MW
Superfamily
SIX/Sine oculis homeobox family
Alternative Names
Homeobox protein SIX6;Homeodomain protein OPTX2;Optic homeobox 2;Sine oculis homeobox homolog 6;SIX6;OPTX2, SIX9; SIX6 MCOPCT2, ODRMD, OPTX2, Six9 SIX homeobox 6 homeobox protein SIX6|homeodomain protein OPTX2|optic homeobox 2|sine oculis homeobox homolog 6|sine oculis homeobox protein 6|sine oculis homeobox-like protein 6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SIX6, check out the SIX6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SIX6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Homeobox protein SIX6 Antibody Picoband® (A06502-1)
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Customer Q&As
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7 Customer Q&As for Anti-Homeobox protein SIX6 Antibody Picoband®
Question
We are currently using anti-Homeobox protein SIX6 antibody A06502-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-09
Answer
The anti-Homeobox protein SIX6 antibody (A06502-1) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-09
Question
Would anti-Homeobox protein SIX6 antibody A06502-1 work for WB with adenohypophysis?
N. Krishna
Verified customer
Asked: 2019-07-29
Answer
According to the expression profile of adenohypophysis, SIX6 is highly expressed in adenohypophysis. So, it is likely that anti-Homeobox protein SIX6 antibody A06502-1 will work for WB with adenohypophysis.
Boster Scientific Support
Answered: 2019-07-29
Question
Would anti-Homeobox protein SIX6 antibody A06502-1 work on horse WB with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2019-03-21
Answer
Our lab technicians have not validated anti-Homeobox protein SIX6 antibody A06502-1 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Homeobox protein SIX6 antibody A06502-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse leukemic t-cell in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-03-21
Question
See below the WB image, lot number and protocol we used for adenohypophysis using anti-Homeobox protein SIX6 antibody A06502-1. Please let me know if you require anything else.
S. Krishna
Verified customer
Asked: 2018-07-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-05
Question
My lab would like to test anti-Homeobox protein SIX6 antibody A06502-1 on human adenohypophysis for research purposes, then I may be interested in using anti-Homeobox protein SIX6 antibody A06502-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-12-12
Answer
The products we sell, including anti-Homeobox protein SIX6 antibody A06502-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-12-12
Question
I see that the anti-Homeobox protein SIX6 antibody A06502-1 works with WB, what is the protocol used to produce the result images on the product page?
E. Brown
Verified customer
Asked: 2016-08-03
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-08-03
Question
I have a question about product A06502-1, anti-Homeobox protein SIX6 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
L. Banerjee
Verified customer
Asked: 2015-07-07
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A06502-1 anti-Homeobox protein SIX6 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-07-07
