Anti-Homeobox protein SIX6 Antibody Picoband™

Product Name

Anti-Homeobox protein SIX6 Antibody Picoband™

View all SIX6 Antibodies

SKU/Catalog Number

A06502-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Homeobox protein SIX6 Antibody Picoband™ catalog # A06502-1. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Homeobox protein SIX6 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06502-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human SIX6 recombinant protein (Position: A188-I246). Human SIX6 shares 91.5% amino acid (aa) sequence identity with mouse SIX6.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A06502-1 is reactive to SIX6 in Human

Applications

A06502-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

28 kDa

Calculated molecular weight

27687 MW

Background of SIX6

The protein encoded by this gene is a homeobox protein that is similar to the Drosophila 'sine oculis' gene product. This gene is found in a cluster of related genes on chromosome 14 and is thought to be involved in eye development. Defects in this gene are a cause of isolated microphthalmia with cataract type 2 (MCOPCT2).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of SIX6 using anti-SIX6 antibody (A06502-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIX6 antigen affinity purified polyclonal antibody (Catalog # A06502-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIX6 at approximately 28KD. The expected band size for SIX6 is at 28KD.

Gene/Protein Information For SIX6 (Source: Uniprot.org, NCBI)

Alternative Names

homeobox protein SIX6; Homeodomain protein OPTX2; MCOPCT2; Optic homeobox 2; OPTX2; sine oculis homeobox (Drosophila) homolog 6; sine oculis homeobox homolog 6 (Drosophila); Sine oculis homeobox homolog 6; SIX homeobox 6; Six9 SIX6 MCOPCT2, ODRMD, OPTX2, Six9 SIX homeobox 6 homeobox protein SIX6|homeodomain protein OPTX2|optic homeobox 2|sine oculis homeobox homolog 6|sine oculis homeobox protein 6|sine oculis homeobox-like protein 6

For more info on SIX6, check out the SIX6 Infographic

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In this infographic, you will see the following information for SIX6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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