Click image to see more details
Product Info Summary
| SKU: | A02688-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-NPAS2 Antibody Picoband®
SKU/Catalog Number
A02688-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NPAS2 Antibody Picoband® catalog # A02688-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NPAS2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02688-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human NPAS2 recombinant protein (Position: D474-Q578). Human NPAS2 shares 88.1% amino acid (aa) sequence identity with mouse NPAS2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02688-1 is reactive to NPAS2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
92 kDa
Calculated molecular weight
91791 MW
Background of NPAS2
Neuronal PAS domain-containing protein 2 is a protein that in humans is encoded by the NPAS2 gene. The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH)-PAS family of transcription factors. A similar mouse protein may play a regulatory role in the acquisition of specific types of memory. It also may function as a part of a molecular clock operative in the mammalian forebrain.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02688-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: rat brain tissue, mouse brain tissue, human COLO-320 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of NPAS2 using anti-NPAS2 antibody (A02688-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysate,
Lane 2: mouse brain tissue lysate,
Lane 3: human COLO-320 whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NPAS2 antigen affinity purified polyclonal antibody (Catalog # A02688-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NPAS2 at approximately 92KD. The expected band size for NPAS2 is at 92KD.
Protein Target Info & Infographic
Gene/Protein Information For NPAS2 (Source: Uniprot.org, NCBI)
Gene Name
NPAS2
Full Name
Neuronal PAS domain-containing protein 2
Weight
91791 MW
Alternative Names
Neuronal PAS domain-containing protein 2;Neuronal PAS2;Basic-helix-loop-helix-PAS protein MOP4;Class E basic helix-loop-helix protein 9;bHLHe9;Member of PAS protein 4;PAS domain-containing protein 4;NPAS2;BHLHE9, MOP4, PASD4; NPAS2 MOP4, PASD4, bHLHe9 neuronal PAS domain protein 2 neuronal PAS domain-containing protein 2|PAS domain-containing protein 4|basic-helix-loop-helix-PAS protein MOP4|class E basic helix-loop-helix protein 9|member of PAS protein 4|member of PAS superfamily 4|neuronal PAS2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NPAS2, check out the NPAS2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NPAS2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NPAS2 Antibody Picoband® (A02688-1)
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Customer Q&As
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3 Customer Q&As for Anti-NPAS2 Antibody Picoband®
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-NPAS2 antibody A02688-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-11-09
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-09
Question
Would anti-NPAS2 antibody A02688-1 work for WB with lung?
Verified Customer
Verified customer
Asked: 2017-11-20
Answer
According to the expression profile of lung, NPAS2 is highly expressed in lung. So, it is likely that anti-NPAS2 antibody A02688-1 will work for WB with lung.
Boster Scientific Support
Answered: 2017-11-20
Question
We are currently using anti-NPAS2 antibody A02688-1 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
S. Li
Verified customer
Asked: 2013-05-29
Answer
The anti-NPAS2 antibody (A02688-1) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-05-29
