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Product Info Summary
| SKU: | PB9357 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-SIRT6 Antibody Picoband®
SKU/Catalog Number
PB9357
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SIRT6 Antibody Picoband® catalog # PB9357. Tested in IHC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SIRT6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9357)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SIRT6 recombinant protein (Position: D14-E180). Human SIRT6 shares 95% amino acid (aa) sequence identity with mouse SIRT6.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9357 is reactive to SIRT6 in Human, Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
39-42 kDa
Calculated molecular weight
39,119 MW
Background of Sirt6
SIRT6 is also known as SIR2L6. This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9357 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human U87 whole cell, human A549 whole cell, human K562 whole cell, human U2OS whole cell, human Hela whole cell
IHC: Mouse Cardiac Muscle Tissue, Human Mammary Cancer Tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SIRT6 using anti-SIRT6 antibody (PB9357).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U87 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human U2OS whole cell lysates,
Lane 5: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIRT6 antigen affinity purified polyclonal antibody (Catalog # PB9357) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIRT6 at approximately 39-42 kDa. The expected band size for SIRT6 is at 39 kDa.
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Anti-SIRT6Picoband antibody, PB9357, IHC(P)
IHC(P): Mouse Cardiac Muscle Tissue
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Anti-SIRT6Picoband antibody, PB9357, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Protein Target Info & Infographic
Gene/Protein Information For SIRT6 (Source: Uniprot.org, NCBI)
Gene Name
SIRT6
Full Name
NAD-dependent protein deacetylase sirtuin-6
Weight
39,119 MW
Superfamily
sirtuin family
Alternative Names
NAD-dependent protein deacetylase sirtuin-6;3.5.1.-;Regulatory protein SIR2 homolog 6;SIR2-like protein 6;SIRT6;SIR2L6; Sirt6|2810449N18Rik, AI043036, Sir2l6|sirtuin 6|NAD-dependent protein deacetylase sirtuin-6|NAD-dependent deacetylase sirtuin-6|SIR2-like protein 6|regulatory protein SIR2 homolog 6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SIRT6, check out the SIRT6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SIRT6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SIRT6 Antibody Picoband® (PB9357)
Hello CJ!
PB9357 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Exogenous Hydrogen Sulfide Ameliorates Diabetic Myocardial Fibrosis by Inhibiting Cell Aging Through SIRT6/AMPK Autophagy
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Customer Q&As
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1 Customer Q&As for Anti-SIRT6 Antibody Picoband®
Question
We are currently using anti-SIRT6 antibody PB9357 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
The anti-SIRT6 antibody (PB9357) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-08
