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Product Info Summary
| SKU: | A02395 |
|---|---|
| Size: | 100μl/vial |
| Reactive Species: | Bovine, Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-SIRT5/Sirtuin 5 Antibody
View all Sirtuin 5/SIRT5 Antibodies
SKU/Catalog Number
A02395
Size
100μl/vial
Form
Liquid
Description
Boster Bio Anti-SIRT5/Sirtuin 5 Antibody (Catalog# A02395). Tested in WB application(s). This antibody reacts with Human, Mouse, Rat, Bovine.
Storage & Handling
Store at -80°C for long-term storage. Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C. (Ship on dry ice.)
Cite This Product
Anti-SIRT5/Sirtuin 5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02395)
Host
Rabbit
Contents
Liquid. In PBS.
Clonality
Polyclonal
Immunogen
Recombinant human SIRT5
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02395 is reactive to SIRT5 in Bovine, Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
33881 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02395 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot (1:2,500, Colorimetric, using secondary antibody coupled to alkaline phosphatase and BCIP/NBT as substrate. / 1:3,000, ECL, using secondary antibody coupled HRP). Detects a band of ~29 kDa by Western blot.
Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.
Validation Images & Assay Conditions
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Western blot analysis of SIRT5 expression in MW marker (lane 1), recombinant human SIRT5 protein (lane 2), recombinant human SIRT3 protein (lane 3), recombinant human SIRT2 protein (lane 4) and recombinant human SIRT1 protein (lane 5). SIRT5 at 29KD was detected using rabbit anti-SIRT5 Partially purified from rabbit serum with immunodepletion polyclonal antibody (Catalog # A02395) at 1: 2500. The blot was developed using colorimetric method.
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Western blot analysis of SIRT5 expression in MW marker (lane 1), recombinant human SIRT5 protein (lane 2), recombinant human SIRT3 protein (lane 3), recombinant human SIRT2 protein (lane 4) and recombinant human SIRT1 protein (lane 5). SIRT5 at 29KD was detected using rabbit anti-SIRT5 Partially purified from rabbit serum with immunodepletion polyclonal antibody (Catalog # A02395) at 1: 3000. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Protein Target Info & Infographic
Gene/Protein Information For SIRT5 (Source: Uniprot.org, NCBI)
Gene Name
SIRT5
Full Name
NAD-dependent protein deacylase sirtuin-5, mitochondrial
Weight
33881 MW
Superfamily
sirtuin family
Alternative Names
NAD-dependent protein deacylase sirtuin-5, mitochondrial ;3.5.1.- ;Regulatory protein SIR2 homolog 5 ;SIR2-like protein 5 ;SIRT5 ;SIR2L5; SIRT5 SIR2L5 sirtuin 5 NAD-dependent protein deacylase sirtuin-5, mitochondrial|NAD-dependent deacetylase sirtuin-5|NAD-dependent lysine demalonylase and desuccinylase sirtuin-5, mitochondrial|silent mating type information regulation 2, S.cerevisiae, homolog 5|sir2-like 5|sirtuin type 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SIRT5, check out the SIRT5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SIRT5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SIRT5/Sirtuin 5 Antibody (A02395)
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No publications found for A02395
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-SIRT5/Sirtuin 5 Antibody
Question
Does anti-SIRT5/Sirtuin 5 antibody A02395 work for WB with testis?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
According to the expression profile of testis, SIRT5 is highly expressed in testis. So, it is likely that anti-SIRT5/Sirtuin 5 antibody A02395 will work for WB with testis.
Boster Scientific Support
Answered: 2020-03-03
Question
Does anti-SIRT5/Sirtuin 5 antibody A02395 work on canine WB with amygdala?
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
Our lab technicians have not tested anti-SIRT5/Sirtuin 5 antibody A02395 on canine. You can run a BLAST between canine and the immunogen sequence of anti-SIRT5/Sirtuin 5 antibody A02395 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine amygdala in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-30
Question
I am interested in to test anti-SIRT5/Sirtuin 5 antibody A02395 on human testis for research purposes, then I may be interested in using anti-SIRT5/Sirtuin 5 antibody A02395 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-13
Answer
The products we sell, including anti-SIRT5/Sirtuin 5 antibody A02395, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-13
Question
I have attached the WB image, lot number and protocol we used for testis using anti-SIRT5/Sirtuin 5 antibody A02395. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-07-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-23
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-SIRT5/Sirtuin 5 antibody A02395. Let me know if you need anything else.
M. Rodriguez
Verified customer
Asked: 2017-02-20
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-02-20
