Click image to see more details
Product Info Summary
| SKU: | PB9161 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-SIRT3 Antibody Picoband®
View all Sirtuin 3/SIRT3 Antibodies
SKU/Catalog Number
PB9161
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SIRT3 Antibody Picoband® catalog # PB9161. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SIRT3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9161)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SIRT3 recombinant protein (Position: P66-K399). Human SIRT3 shares 86% amino acid (aa) sequence identity with mouse SIRT3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9161 is reactive to SIRT3 in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
44 kDa
Calculated molecular weight
43573 MW
Background of Sirtuin 3/SIRT3
NAD-dependent deacetylase sirtuin-3, mitochondrial also known as SIRT3 is a protein that in humans is encoded by the SIRT3 gene. SIRT3 is member of the mammalian sirtuin family of proteins, which are homologs to the yeast Sir2 protein. It is mapped to 11p15.5. Endogenous SIRT3 is a soluble protein located in the mitochondrial matrix. Overexpression of SIRT3 in cultured cells increases respiration and decreases the production of reactive oxygen species. SIRT3 can function as a mitochondrial deacetylase. In addition to it, human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9161 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: rat spleen tissue, human placenta tissue, human Hela whole cell, human COLO320 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of SIRT3 using anti-SIRT3 antibody (PB9161).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant human SIRT3 protein 0.5 ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIRT3 antigen affinity purified polyclonal antibody (Catalog # PB9161) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIRT3 at approximately 39 kDa. The expected band size for SIRT3 is at 39 kDa.
Click image to see more details
Figure 2. Western blot analysis of SIRT3 using anti-SIRT3 antibody (PB9161).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,
Lane 2: human placenta tissue lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human COLO320 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIRT3 antigen affinity purified polyclonal antibody (Catalog # PB9161) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIRT3 at approximately 44 kDa. The expected band size for SIRT3 is at 44 kDa.
Protein Target Info & Infographic
Gene/Protein Information For SIRT3 (Source: Uniprot.org, NCBI)
Gene Name
SIRT3
Full Name
NAD-dependent protein deacetylase sirtuin-3, mitochondrial
Weight
43573 MW
Superfamily
sirtuin family
Alternative Names
NAD-dependent protein deacetylase sirtuin-3, mitochondrial;hSIRT3;3.5.1.-;Regulatory protein SIR2 homolog 3;SIR2-like protein 3;SIRT3;SIR2L3; SIRT3 SIR2L3 sirtuin 3 NAD-dependent protein deacetylase sirtuin-3, mitochondrial|NAD-dependent deacetylase sirtuin-3, mitochondrial|SIR2-like protein 3|mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase|regulatory protein SIR2 homolog 3|silent mating type information regulation 2, S.cerevisiae, homolog 3|sir2-like 3|sirtuin type 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SIRT3, check out the SIRT3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SIRT3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SIRT3 Antibody Picoband® (PB9161)
Hello CJ!
No publications found for PB9161
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-SIRT3 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-SIRT3 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
15 Customer Q&As for Anti-SIRT3 Antibody Picoband®
Question
I see that the anti-SIRT3 antibody PB9161 works with WB, what is the protocol used to produce the result images on the product page?
J. Li
Verified customer
Asked: 2020-03-20
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-03-20
Question
you antibody to test anti-SIRT3 antibody PB9161 on rat tongue for research purposes, then I may be interested in using anti-SIRT3 antibody PB9161 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
The products we sell, including anti-SIRT3 antibody PB9161, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-13
Question
Is this PB9161 anti-SIRT3 antibody reactive to the isotypes of SIRT3?
Verified Customer
Verified customer
Asked: 2020-01-14
Answer
The immunogen of PB9161 anti-SIRT3 antibody is E.coli-derived human SIRT3 recombinant protein (Position: P66-K399). Human SIRT3 shares 86% amino acid (aa) sequence identity with mouse SIRT3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-14
Question
Will anti-SIRT3 antibody PB9161 work for WB with tongue?
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
According to the expression profile of tongue, SIRT3 is highly expressed in tongue. So, it is likely that anti-SIRT3 antibody PB9161 will work for WB with tongue.
Boster Scientific Support
Answered: 2020-01-06
Question
Is a blocking peptide available for product anti-SIRT3 antibody (PB9161)?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
We do provide the blocking peptide for product anti-SIRT3 antibody (PB9161). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-02
Question
I was wanting to use your anti-SIRT3 antibody for WB for rat tongue on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat tongue identification?
Verified Customer
Verified customer
Asked: 2019-10-18
Answer
You can see on the product datasheet, PB9161 anti-SIRT3 antibody has been tested for WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat tongue in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-18
Question
Does PB9161 anti-SIRT3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
You can see on the product datasheet, PB9161 anti-SIRT3 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-29
Question
See below the WB image, lot number and protocol we used for tongue using anti-SIRT3 antibody PB9161. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-15
Question
Do you have a BSA free version of anti-SIRT3 antibody PB9161 available?
Verified Customer
Verified customer
Asked: 2019-05-14
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-SIRT3 antibody PB9161 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-14
Question
We want using your anti-SIRT3 antibody for aging studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-12-19
Answer
We appreciate your inquiry. This PB9161 anti-SIRT3 antibody is validated on human placenta tissue, rat spleen tissue, tissue lysate, hela whole cell lysate, colo320 whole cell lysate. It is guaranteed to work for WB in human, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-12-19
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for tongue using anti-SIRT3 antibody PB9161. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-05-24
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-24
Question
Can you help my question with product PB9161, anti-SIRT3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-04-19
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9161 anti-SIRT3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-04-19
Question
We are currently using anti-SIRT3 antibody PB9161 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2017-10-31
Answer
The anti-SIRT3 antibody (PB9161) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-10-31
Question
My boss were content with the WB result of your anti-SIRT3 antibody. However we have seen positive staining in tongue mitochondrion matrix using this antibody. Is that expected? Could you tell me where is SIRT3 supposed to be expressed?
K. Dhar
Verified customer
Asked: 2015-03-30
Answer
According to literature, tongue does express SIRT3. Generally SIRT3 expresses in mitochondrion matrix. Regarding which tissues have SIRT3 expression, here are a few articles citing expression in various tissues:
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 15489334
Testis, Pubmed ID: 10381378, 17974005
Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2015-03-30
Question
We have seen staining in human tongue. What should we do? Is anti-SIRT3 antibody supposed to stain tongue positively?
N. Jackson
Verified customer
Asked: 2014-06-26
Answer
From literature tongue does express SIRT3. From Uniprot.org, SIRT3 is expressed in anterior cingulate cortex, testis, tongue, lung, leukemic t-cell, liver, among other tissues. Regarding which tissues have SIRT3 expression, here are a few articles citing expression in various tissues:
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 15489334
Testis, Pubmed ID: 10381378, 17974005
Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2014-06-26
