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Product Info Summary
| SKU: | M05151 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-SIRP alpha (SIRPA) Mouse Monoclonal Antibody [Clone ID: OTI7B3]
View all SIRP alpha/CD172a Antibodies
SKU/Catalog Number
M05151
Size
100 µl
Description
Boster Bio SIRPA mouse monoclonal antibody, clone OTI7B3 (formerly 7B3). Catalog# M05151. Tested in FC, IHC, WB. This antibody reacts with Human.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-SIRP alpha (SIRPA) Mouse Monoclonal Antibody [Clone ID: OTI7B3] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05151)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI7B3
Isotype
IgG1
Immunogen
Full length human recombinant protein of human SIRPA (NP_001035111) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M05151 is reactive to SIRPA in Human
Reconstitution
Calculated molecular weight
56.414kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05151 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIRPA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIRPA (1:2000).
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-SIRPA mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SIRPA mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA
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Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-SIRPA mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA
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Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-SIRPA mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-SIRPA mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA
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Flow cytometric analysis of living ACHN cells
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Flow cytometric analysis of living A375 cells
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Flow cytometric analysis of living 293T cells transfected with SIRPA overexpression plasmid
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Flow cytometric analysis of living SK-MEL-28 cells
Protein Target Info & Infographic
Gene/Protein Information For SIRPA (Source: Uniprot.org, NCBI)
Gene Name
SIRPA
Full Name
Tyrosine-protein phosphatase non-receptor type substrate 1
Weight
56.414kDa
Alternative Names
BIT; BITbrain-immunoglobulin-like molecule with tyrosine-based activation motifs; Brain Ig-like molecule with tyrosine-based activation motifs; CD172 antigen-like family member A; CD172a antigen; CD172a; Inhibitory receptor SHPS-1; Macrophage fusion receptor; MFR; MFRtyrosine phosphatase SHP substrate 1; MyD-1 antigen; MYD1; MYD-1; P84; protein tyrosine phosphatase, non-receptor type substrate 1; PTPNS1; SHP substrate 1; SHPS1; SHPS-1; SHPS1CD172A; signal-regulatory protein alpha; Signal-regulatory protein alpha-1; Signal-regulatory protein alpha-2; Signal-regulatory protein alpha-3; SIRP alph Sirpa|AI835480, Bi, Bit, CD172, CD172a, P8, P84, Pt, Ptpns1, SHP, SHP-1, SHPS-1, SIRP|signal-regulatory protein alpha|tyrosine-protein phosphatase non-receptor type substrate 1|CD172 -like family member A|SHP substrate 1|brain Ig-like molecule with tyrosine-based activation motifs|brain immunological-like with tyrosine-based motifs|inhibitory receptor SHPS-1|mSIRP-alpha1|myD-1 |protein tyrosine phosphatase, non-receptor type substrate 1|signal-regulatory protein alpha-1|sirp-alpha-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SIRPA, check out the SIRPA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SIRPA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SIRP alpha (SIRPA) Mouse Monoclonal Antibody [Clone ID: OTI7B3] (M05151)
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