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Product Info Summary
| SKU: | A05151 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, ICC, WB |
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Product info
Product Name
Anti-SIRP alpha Antibody
View all SIRP alpha/CD172a Antibodies
SKU/Catalog Number
A05151
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SIRP alpha Antibody (Catalog # A05151). Tested in ELISA, WB, ICC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
SIRP alpha antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-SIRP alpha Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05151)
Host
Rabbit
Contents
SIRP alpha Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
SIRP alpha antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human SIRP alpha 1. The immunogen is located within the last 50 amino acids of SIRP alpha.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Recognizes SIRP alpha 1, 2 and 3.
Reactive Species
A05151 is reactive to SIRPA in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
54967 MW
Background of SIRP alpha/CD172a
Protein tyrosine phosphatases (PTPases) SHP-1 and SHP-2 are critical regulators in the intracellular signaling pathways that result in cell responses such as mitosis, differentiation, migration, survival, transformation or death. SHP-2 is a signal transducer for several receptor tyrosine kinases and cytokine receptors. A novel SHP-2 associated glycoprotein was recently cloned from human, rat, mouse and cattle by several labs and was designated SIRPalpha, SHPS-1, MyD-1, BIT and p84. SIRPalpha is a new gene family containing at least fifteen members. SIRPalpha is a substrate of many activated tyrosine kinases such as insulin receptor, EGFR, PDGFR and src, and a specific docking protein for SHP-2. SIRPalpha has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion and plays a general role in different physiological and pathological processes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05151 is guaranteed for ELISA, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SIRP alpha antibody can be used for Western blot at 0.5 - 1 μg/mL. 75 - 110 kDa bands should be detected. Antibody can also be used for immunocytochemistry starting at 1 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SIRP alpha in THP-1 whole cell lysate with SIRP alpha antibody at 0.5 μg/mL.
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Immunocytochemistry of SIRP alpha in THP-1 cells with SIRP alpha antibody at 1 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For SIRPA (Source: Uniprot.org, NCBI)
Gene Name
SIRPA
Full Name
Tyrosine-protein phosphatase non-receptor type substrate 1
Weight
54967 MW
Alternative Names
BIT, MFR, P84, SIRP, MYD-1, SHPS1, CD172A, PTPNS1, BIT, MYD1, Tyrosine-protein phosphatase non-receptor type substrate 1, Brain Ig-like molecule with tyrosine-based activation motifs, SHP substrate 1 Sirpa|AI835480, Bi, Bit, CD172, CD172a, P8, P84, Pt, Ptpns1, SHP, SHP-1, SHPS-1, SIRP|signal-regulatory protein alpha|tyrosine-protein phosphatase non-receptor type substrate 1|CD172 -like family member A|SHP substrate 1|brain Ig-like molecule with tyrosine-based activation motifs|brain immunological-like with tyrosine-based motifs|inhibitory receptor SHPS-1|mSIRP-alpha1|myD-1 |protein tyrosine phosphatase, non-receptor type substrate 1|signal-regulatory protein alpha-1|sirp-alpha-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SIRPA, check out the SIRPA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SIRPA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SIRP alpha Antibody (A05151)
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