Anti-SIK1 Antibody

Product Name

Anti-SIK1 Antibody

View all SIK1/Snf1lk Antibodies

SKU/Catalog Number

A04399

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-SIK1 Antibody (Catalog # A04399). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human.

Storage & Handling

SIK1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-SIK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04399)

Host

Rabbit

Contents

SIK1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

SIK1 antibody was raised against an 18 amino acid synthetic peptide near the center of human SIK1. The immunogen is located within amino acids 340 - 390 of SIK1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Two isoforms of SIK1 are known to exist; this antibody will detect both isoforms. SIK1 antibody is predicted to not cross-react with other SIK protein family members.

Reactive Species

A04399 is reactive to SIK1 in Human

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

84902 MW

Background of SIK1/Snf1lk

Salt-inducible kinase 1 (SIK1), also known as SNF1LK or MSK, plays a role in histone modification and G2/M cell cycle regulation. It is a 783 amino acid protein that contains one UBA domain and belongs to the Ser/Thr protein kinase family (AMPK subfamily). Localized to both the nucleus and the cytoplasm, SIK1 is a class II HDAC kinase that uses magnesium as a cofactor to catalyze the ATP-dependent phosphorylation of target proteins and is thought to be important for the early stages of skeletal muscle growth and myocardial cell differentiation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A04399 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

SIK1 antibody can be used for detection of SIK1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For SIK1 (Source: Uniprot.org, NCBI)

Alternative Names

MSK, SIK, SNF1LK, Salt-inducible kinase 1, SIK-1 Sik1|Hrt-20, Ms, Msk, Sik, Sik-1, Snf, Snf1lk|salt inducible kinase 1|serine/threonine-protein kinase SIK1|Probable serine/threonine protein kinase SNF1LK (HRT-20) (Myocardial SNF1-like kinase)|SNF1-like kinase|myocardial SNF1-like kinase|salt-inducible protein kinase 1|serine/threonine-protein kinase SNF1-like kinase 1|serine/threonine-protein kinase SNF1LK

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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