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Product Info Summary
| SKU: | PB9326 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Sex Hormone Binding Globulin/SHBG Antibody Picoband®
SKU/Catalog Number
PB9326
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Sex Hormone Binding Globulin/SHBG Antibody Picoband® catalog # PB9326. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Sex Hormone Binding Globulin/SHBG Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9326)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SHBG recombinant protein (Position: L30-A210). Human SHBG shares 72% amino acid (aa) sequence identity with both mouse and rat SHBG.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9326 is reactive to SHBG in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
70 kDa
Calculated molecular weight
43779 MW
Background of SHBG
Sex hormone-binding globulin (SHBG) is a glycoprotein that binds to the sex hormones,androgen and estrogen. SHBG is produced mostly by the liver and is released into the bloodstream. Other sites that produce SHBG include the brain, uterus, testes, and placenta. SHBG has both enhancing and inhibiting hormonal influences. It decreases with high levels of insulin, growth hormone, insulin-like growth factor 1 (IGF-1), androgens, prolactin and transcortin. High estrogen, andthyroxine cause it to increase. It has been found that the monosaccharides glucose and fructose reduced human SHBG production by hepatocytes, irrespective of the presence of insulin, by downregulation of hepatocyte nuclear factor-4A and replacement of HNF4A by COUP-TFI at a cis-element within the human SHBG promoter, coincident with repression of its transcriptional activity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9326 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: HELA Whole Cell, COLO320 Whole Cell
Validation Images & Assay Conditions
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Anti-SHBG Picoband antibody, PB9326, Western blotting
All lanes: Anti SHBG (PB9326) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 44KD
Observed bind size: 70KD
Protein Target Info & Infographic
Gene/Protein Information For SHBG (Source: Uniprot.org, NCBI)
Gene Name
SHBG
Full Name
Sex hormone-binding globulin
Weight
43779 MW
Alternative Names
Sex hormone-binding globulin;SHBG;Sex steroid-binding protein;SBP;Testis-specific androgen-binding protein;ABP;Testosterone-estradiol-binding globulin;TeBG;Testosterone-estrogen-binding globulin;SHBG; SHBG ABP, SBP, TEBG sex hormone binding globulin sex hormone-binding globulin|sex steroid-binding protein|testis-specific androgen-binding protein|testosterone-binding beta-globulin|testosterone-estradiol-binding globulin|testosterone-estrogen-binding globulin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SHBG, check out the SHBG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SHBG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Sex Hormone Binding Globulin/SHBG Antibody Picoband® (PB9326)
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No publications found for PB9326
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Customer Q&As
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4 Customer Q&As for Anti-Sex Hormone Binding Globulin/SHBG Antibody Picoband®
Question
We want to test anti-Sex Hormone Binding Globulin/SHBG antibody PB9326 on human plasma for research purposes, then I may be interested in using anti-Sex Hormone Binding Globulin/SHBG antibody PB9326 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-03-22
Answer
The products we sell, including anti-Sex Hormone Binding Globulin/SHBG antibody PB9326, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-03-22
Question
Does anti-Sex Hormone Binding Globulin/SHBG antibody PB9326 work for WB with plasma?
Verified Customer
Verified customer
Asked: 2017-06-16
Answer
According to the expression profile of plasma, SHBG is highly expressed in plasma. So, it is likely that anti-Sex Hormone Binding Globulin/SHBG antibody PB9326 will work for WB with plasma.
Boster Scientific Support
Answered: 2017-06-16
Question
We are currently using anti-Sex Hormone Binding Globulin/SHBG antibody PB9326 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on zebrafish tissues as well?
V. Patel
Verified customer
Asked: 2017-01-20
Answer
The anti-Sex Hormone Binding Globulin/SHBG antibody (PB9326) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-01-20
Question
Does anti-Sex Hormone Binding Globulin/SHBG antibody PB9326 work on goat WB with liver?
A. Mangal
Verified customer
Asked: 2016-04-01
Answer
Our lab technicians have not tested anti-Sex Hormone Binding Globulin/SHBG antibody PB9326 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Sex Hormone Binding Globulin/SHBG antibody PB9326 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-04-01
