Product Info Summary
SKU: | A17230 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-SH3TC1 Antibody
SKU/Catalog Number
A17230
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-SH3TC1 Antibody (Catalog # A17230). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SH3TC1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A17230)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human SH3TC1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A17230 is reactive to SH3TC1 in Human
Reconstitution
Calculated molecular weight
146961 MW
Background of SH3TC1
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a putative 39S subunit protein and belongs to the L47P ribosomal protein family. Pseudogenes corresponding to this gene are found on chromosomes 2p, 2q, 5p, and 10q.
Ota T., Nat. Genet. 36:40-45(2004).
Humphray S.J., Nature 429:369-374(2004).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A17230 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from K562 cells, using SH3TC1 antibody A17230.
Protein Target Info & Infographic
Gene/Protein Information For SH3TC1 (Source: Uniprot.org, NCBI)
Gene Name
SH3TC1
Full Name
SH3 domain and tetratricopeptide repeat-containing protein 1
Weight
146961 MW
Alternative Names
SH3 domain and tetratricopeptide repeat-containing protein 1;SH3TC1; SH3TC1 SH3 domain and tetratricopeptide repeats 1 SH3 domain and tetratricopeptide repeat-containing protein 1|SH3 domain and tetratricopeptide repeats-containing protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SH3TC1, check out the SH3TC1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SH3TC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SH3TC1 Antibody (A17230)
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No publications found for A17230
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Customer Q&As
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5 Customer Q&As for Anti-SH3TC1 Antibody
Question
Will A17230 anti-SH3TC1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-25
Answer
It shows on the product datasheet, A17230 anti-SH3TC1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-25
Question
We are currently using anti-SH3TC1 antibody A17230 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-15
Answer
The anti-SH3TC1 antibody (A17230) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-15
Question
Is there a BSA free version of anti-SH3TC1 antibody A17230 available?
Verified Customer
Verified customer
Asked: 2018-10-04
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-SH3TC1 antibody A17230 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-10-04
Question
I was wanting to use your anti-SH3TC1 antibody for WB for human leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human leukemic t-cell identification?
J. Moore
Verified customer
Asked: 2013-07-25
Answer
As indicated on the product datasheet, A17230 anti-SH3TC1 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-07-25
Question
you antibody to test anti-SH3TC1 antibody A17230 on human leukemic t-cell for research purposes, then I may be interested in using anti-SH3TC1 antibody A17230 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
G. Baker
Verified customer
Asked: 2013-01-30
Answer
The products we sell, including anti-SH3TC1 antibody A17230, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-01-30