Anti-Phospho-SH3BP2 S427 Antibody

Product Name

Anti-Phospho-SH3BP2 S427 Antibody

View all SH3BP2 Antibodies

SKU/Catalog Number

P04008

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Phospho-SH3BP2 S427 Antibody (Catalog # P04008). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-Phospho-SH3BP2 S427 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P04008)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 415-440 of Human SH3BP3 protein (SH3 Domain Binding Protein 2).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P04008 is reactive to SH3BP2 in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

42 kDa

Calculated molecular weight

62244 MW

Background of SH3BP2

SH3BP2 Src Homology 3 Binding Protein 2 is also known as 3BP-2 and SH3 Binding Protein 2. The Src homology 3 (SH3) region is a small protein domain presented in a very large group of proteins, including cytoskeletal elements and signaling proteins. SH3 domains are believed to serve as modules that mediate protein-protein associations and, along with Src homology 2 (SH2) domains, regulate cytoplasmic signaling. SH3BP2 is composed of an N terminal pleckstrin homology (PH) domain, a ten aa SH3 binding domain, three modular peptide recognition domains, and a C terminal SH2 domain. SH3BP2 function relates to signal transduction and regulation. SH3BP2 binds differentially to the SH3 domains of certain proteins of signal transduction pathways. Phosphorylation of SH3BP2 occurs on S427 for activation. SH3BP2 mediates interactions of huntingtin and MLK2 (mixed lineage kinase). Defects in SH3BP2 are the cause of cherubism (CRBM), an autosomal dominant inherited syndrome. It is characterized by excessive bone degradation of the upper and lower jaws, which often begins around three years of age. It is followed by development of fibrous tissue masses, which causes a characteristic facial swelling.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

P04008 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:2,000 - 1:10,000
IP: 1:100
WB: 1:500 - 1:2,000

Validation Images & Assay Conditions

Gene/Protein Information For SH3BP2 (Source: Uniprot.org, NCBI)

Alternative Names

SH3BP2 pS427, phosphorylated SH3BP2, SH3 domain-binding protein 2, SH3BP2 Src homology 3 Binding Protein 2, 3BP-2, RES4-23, 3BP2 SH3BP2 3BP-2, 3BP2, CRBM, CRPM, RES4-23 SH3 domain binding protein 2 SH3 domain-binding protein 2|Abl-SH3 binding protein 2|TNFAIP3 interacting protein 2|epididymis secretory sperm binding protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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