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Product Info Summary
| SKU: | A04093 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-NDUFS5 Antibody
SKU/Catalog Number
A04093
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-NDUFS5 Antibody (Catalog # A04093). Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NDUFS5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04093)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human NDUFS5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04093 is reactive to NDUFS5 in Human
Reconstitution
Calculated molecular weight
12518 MW
Background of NDUFS5
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Mao M., Proc. Natl. Acad. Sci. U.S.A. 95:8175-8180(1998).
Loeffen J., J. Inherit. Metab. Dis. 22:19-28(1999).<
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04093 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Western blot analysis of extracts from K562 cells, using NDUFS5 antiobdy A04093.
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Immunohistochemistry analysis of paraffin-embedded human heart tissue using NDUFS5 antibody A04093.
Protein Target Info & Infographic
Gene/Protein Information For NDUFS5 (Source: Uniprot.org, NCBI)
Gene Name
NDUFS5
Full Name
NADH dehydrogenase [ubiquinone] iron-sulfur protein 5
Weight
12518 MW
Superfamily
complex I NDUFS5 subunit family
Alternative Names
NADH dehydrogenase [ubiquinone] iron-sulfur protein 5;Complex I-15 kDa;CI-15 kDa;NADH-ubiquinone oxidoreductase 15 kDa subunit;NDUFS5; NDUFS5 CI-15k, CI15K NADH:ubiquinone oxidoreductase subunit S5 NADH dehydrogenase [ubiquinone] iron-sulfur protein 5|CI-15 kDa|NADH dehydrogenase (ubiquinone) Fe-S protein 5, 15kDa (NADH-coenzyme Q reductase)|NADH:ubiquinone oxidoreductase 15 kDa IP subunit|complex I-15 kDa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NDUFS5, check out the NDUFS5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NDUFS5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NDUFS5 Antibody (A04093)
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No publications found for A04093
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-NDUFS5 Antibody
Question
My question regarding product A04093, anti-NDUFS5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-06
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04093 anti-NDUFS5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-06
Question
Is this A04093 anti-NDUFS5 antibody reactive to the isotypes of NDUFS5?
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
The immunogen of A04093 anti-NDUFS5 antibody is Synthesized peptide derived from internal of human NDUFS5. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-27
Question
Will anti-NDUFS5 antibody A04093 work on pig WB with liver?
Verified Customer
Verified customer
Asked: 2020-03-06
Answer
Our lab technicians have not tested anti-NDUFS5 antibody A04093 on pig. You can run a BLAST between pig and the immunogen sequence of anti-NDUFS5 antibody A04093 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-06
Question
I was wanting to use your anti-NDUFS5 antibody for WB for human umbilical cord blood on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human umbilical cord blood identification?
Verified Customer
Verified customer
Asked: 2019-11-04
Answer
You can see on the product datasheet, A04093 anti-NDUFS5 antibody has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human umbilical cord blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-04
Question
I have attached the WB image, lot number and protocol we used for umbilical cord blood using anti-NDUFS5 antibody A04093. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-28
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-28
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for umbilical cord blood using anti-NDUFS5 antibody A04093. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-17
Question
Do you have a BSA free version of anti-NDUFS5 antibody A04093 available?
Verified Customer
Verified customer
Asked: 2019-09-03
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-NDUFS5 antibody A04093 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-03
Question
Would A04093 anti-NDUFS5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-13
Answer
As indicated on the product datasheet, A04093 anti-NDUFS5 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-13
Question
Would anti-NDUFS5 antibody A04093 work for WB with umbilical cord blood?
M. Wu
Verified customer
Asked: 2018-11-14
Answer
According to the expression profile of umbilical cord blood, NDUFS5 is highly expressed in umbilical cord blood. So, it is likely that anti-NDUFS5 antibody A04093 will work for WB with umbilical cord blood.
Boster Scientific Support
Answered: 2018-11-14
Question
Is a blocking peptide available for product anti-NDUFS5 antibody (A04093)?
T. Jackson
Verified customer
Asked: 2018-11-01
Answer
We do provide the blocking peptide for product anti-NDUFS5 antibody (A04093). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-11-01
Question
I am interested in to test anti-NDUFS5 antibody A04093 on human umbilical cord blood for research purposes, then I may be interested in using anti-NDUFS5 antibody A04093 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-02-23
Answer
The products we sell, including anti-NDUFS5 antibody A04093, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-02-23
Question
I see that the anti-NDUFS5 antibody A04093 works with WB, what is the protocol used to produce the result images on the product page?
W. Parker
Verified customer
Asked: 2017-12-15
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-12-15
Question
We are currently using anti-NDUFS5 antibody A04093 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on primate tissues as well?
K. Wu
Verified customer
Asked: 2016-09-30
Answer
The anti-NDUFS5 antibody (A04093) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-09-30
