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Product Info Summary
| SKU: | A00673-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-SGK1 Antibody
SKU/Catalog Number
A00673-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SGK1 Antibody (Catalog # A00673-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
SGK1 antibody can be stored at 4 °C for 3 months and -20 °C stable for one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-SGK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00673-1)
Host
Rabbit
Contents
SGK1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
SGK1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SGK1. The immunogen is located within amino acids 370 - 420 of SGK1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
SGK1 antibody is predicted to not cross-react with other SGK protein family members. At least five isoforms are known to exist
Reactive Species
A00673-1 is reactive to SGK1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
48942 MW
Background of SGK1
Serum and glucocorticoid-regulated protein kinase 1 (SGK1) is a member of the AGC family of serine/threonine kinases that plays an important role in cellular stress response and cell survival. SGK1 is transcriptionally activated by numerous stimuli, including serum, glucocorticoids, osmotic stress, and hormones. SGK1 functions as a regulator of cell survival and ion channels and transporters such as ENaC or KCNA3/Kv1.3. It phosphorylates and negatively regulates pro-apoptotic FOXO3A. SGK1 is thought to play an essential function in nephropathy and cardiovascular development.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00673-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SGK1 antibody can be used for detection of SGK1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SGK1 in human stomach tissue lysate with SGK1 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of SGK1 in human stomach tissue with SGK1 antibody at 5 μg/mL.
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Immunofluorescence of SGK1 in human stomach tissue with SGK1 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For SGK1 (Source: Uniprot.org, NCBI)
Gene Name
SGK1
Full Name
Serine/threonine-protein kinase Sgk1
Weight
48942 MW
Superfamily
protein kinase superfamily
Alternative Names
SGK, SGK SGK1 SGK serum/glucocorticoid regulated kinase 1 serine/threonine-protein kinase Sgk1|Sgk1 variant i3|serine/threonine protein kinase SGK
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SGK1, check out the SGK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SGK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SGK1 Antibody (A00673-1)
Hello CJ!
A00673-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Liu BW,Zhang J,Hong YS,Li NB,Liu Y,Zhang M,Wu WY,Zheng H,Lampert A,Zhang XW.NGF-Induced Nav1.7 Upregulation Contributes to Chronic Post-surgical Pain by Activating SGK1-Dependent Nedd4-2 Phosphorylation.Mol Neurobiol.2020 Oct 16.doi:10.1007/s12035-020-021
Species: Mouse,Rat
A00673-1 usage in article: APP:IHC, SAMPLE:SPINAL CORD TISSUE, DILUTION:1:100
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