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Product Info Summary
| SKU: | A07683-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-SFR1 Antibody Picoband®
SKU/Catalog Number
A07683-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SFR1 Antibody Picoband® catalog # A07683-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SFR1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07683-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human SFR1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A07683-1 is reactive to SFR1 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
22 kDa
Calculated molecular weight
32679 MW
Background of MEIR5
SFR1 is a component of the SWI5-SFR1 complex, a complex required for double-strand break repair via homologous recombination.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07683-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
Positive Control
WB: human A431 whole cell, human U251 whole cell, human Hacat whole cell, human Jurkat whole cell, rat liver tissue, mouse brain tissue, mouse liver tissue
FCM: K562 cell, U251 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SFR1 using anti-SFR1 antibody (A07683-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human U251 whole cell lysates,
Lane 3: human Hacat whole cell lysates,
Lane 4: human Jurkat whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFR1 antigen affinity purified polyclonal antibody (Catalog # A07683-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SFR1 at approximately 22 kDa. The expected band size for SFR1 is at 28 kDa.
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Figure 2. Flow Cytometry analysis of U251 cells using anti-SFR1 antibody (A07683-1).
Overlay histogram showing U251 cells stained with A07683-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SFR1 Antibody (A07683-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For SFR1 (Source: Uniprot.org, NCBI)
Gene Name
SFR1
Full Name
Swi5-dependent recombination DNA repair protein 1 homolog
Weight
32679 MW
Superfamily
SFR1/MEI5 family
Alternative Names
Trem-like transcript 1 protein;TLT-1;Triggering receptor expressed on myeloid cells-like protein 1;TREML1;TLT1;UNQ1825/PRO3438; Sfr1|6330577E15Rik, Meir5|SWI5 dependent recombination repair 1|swi5-dependent recombination DNA repair protein 1 homolog|DNA repair protein Sfr1|meiosis protein 5 homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SFR1, check out the SFR1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SFR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SFR1 Antibody Picoband® (A07683-1)
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