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Product Info Summary
| SKU: | A12342-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-SEPTIN12 Antibody Picoband®
SKU/Catalog Number
A12342-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SEPTIN12 Antibody Picoband® catalog # A12342-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SEPTIN12 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12342-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SEPTIN12 recombinant protein (Position: K71-F358).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A12342-2 is reactive to SEPTIN12 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
45 kDa
Calculated molecular weight
40.748kDa
Background of SEPTIN12
Septin 12 is a protein that in humans is encoded by the SEPT12 gene. This gene encodes a guanine-nucleotide binding protein and member of the septin family of cytoskeletal GTPases. Septins play important roles in cytokinesis, exocytosis, embryonic development, and membrane dynamics. Multiple transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12342-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Mouse, Rat
ELISA, 0.1-0.5 µg/ml, Human
Positive Control
WB: rat brain tissue, rat testis tissue, mouse brain tissue, mouse testis tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SEPTIN12 using anti-SEPTIN12 antibody (A12342-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat testis tissue lysates,
Lane 3: mouse brain tissue lysates,
Lane 4: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEPTIN12 antigen affinity purified polyclonal antibody (Catalog # A12342-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEPTIN12 at approximately 45 kDa. The expected band size for SEPTIN12 is at 41 kDa.
Protein Target Info & Infographic
Gene/Protein Information For SEPTIN12 (Source: Uniprot.org, NCBI)
Gene Name
SEPTIN12
Full Name
Septin-12
Weight
40.748kDa
Superfamily
TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily
Alternative Names
Mitochondrial import inner membrane translocase subunit Tim17-A; Inner membrane preprotein translocase Tim17a; TIMM17A; MIMT17; TIM17; TIM17A; TIMM17 SEPTIN12 SEPT12, SPGF10 septin 12 septin-12|testicular tissue protein Li 168
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SEPTIN12, check out the SEPTIN12 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SEPTIN12: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SEPTIN12 Antibody Picoband® (A12342-2)
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