Product Info Summary
SKU: | A30760-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-Septin 8/Septin8 Antibody Picoband®
SKU/Catalog Number
A30760-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Septin 8/Septin8 Antibody Picoband® catalog # A30760-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Septin 8/Septin8 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30760-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse Septin 8/Septin8, which shares 95.7% and 100% amino acid (aa) sequence identity with human and rat SEPTIN8, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A30760-1 is reactive to Septin8 in Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
56 kDa
Calculated molecular weight
14377 MW
Background of Septin8
Predicted to enable GTPase activity; SNARE binding activity; and molecular adaptor activity. Predicted to be involved in several processes, including regulation of SNARE complex assembly; regulation of intracellular protein transport; and regulation of protein stability. Located in myelin sheath. Part of septin complex. Is expressed in dermis; spinal cord ventricular layer; turbinate bone primordium; and ventral grey horn. Orthologous to human Septin8 (septin 8).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30760-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Mouse
Positive Control
WB: rat C6 whole cell, mouse brain tissue
FCM: RAW2647 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Septin 8/Septin8 using anti-Septin 8/Septin8 antibody (A30760-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat C6 whole cell lysates,
Lane 2: mouse brain tissue lysates.
red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Septin 8/Septin8 antigen affinity purified polyclonal antibody (Catalog # A30760-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Septin 8/Septin8 at approximately 56 kDa. The expected band size for Septin 8/Septin8 is at 56 kDa.
Click image to see more details
Figure 2. Flow Cytometry analysis of RAW264.7 cells using anti-Septin 8/Septin8 antibody (A30760-1).
Overlay histogram showing RAW264.7 cells stained with A30760-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Septin 8/Septin8 Antibody (A30760-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For Septin8 (Source: Uniprot.org, NCBI)
Gene Name
Septin8
Full Name
Septin-8
Weight
14377 MW
Superfamily
TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily
Alternative Names
Lymphocyte antigen 6A-2/6E-1;Ly-6A.2/Ly-6E.1;Stem cell antigen 1;SCA-1;T-cell-activating protein;TAP;Ly6a;Ly6; Septin8|AW046166, S, Sep, Sepl, Sept8|septin 8|septin-8
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Septin8, check out the Septin8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Septin8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Septin 8/Septin8 Antibody Picoband® (A30760-1)
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