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Product Info Summary
| SKU: | A04364-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Separase (A795) ESPL1 Antibody
SKU/Catalog Number
A04364-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Separase (A795) ESPL1 Antibody catalog # A04364-1. Tested in WB,IHC,IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Separase (A795) ESPL1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04364-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide, corresponding to amino acids 763-824 of Human Separase.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04364-1 is reactive to ESPL1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
233175 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04364-1 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
IF: 1:50-1:200
Validation Images & Assay Conditions
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Figure 2. Immunohistochemistry validation of ESPL1 using Anti-Separase (A795) ESPL1 Antibody (A04364-1).
Immunohistochemistry (IHC) analyzes of Separase (A795) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.For more protocol information of IHC
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Figure 1. Western blotting validation for Anti-Separase (A795) ESPL1 Antibody A04364-1
Western blot (WB) analysis of Separase (A795) polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysate
Lane2:sp2/0 whole cell lysate
Lane3:PC12 whole cell lysate
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For ESPL1 (Source: Uniprot.org, NCBI)
Gene Name
ESPL1
Full Name
Separin
Weight
233175 MW
Alternative Names
Separin;3.4.22.49;Caspase-like protein ESPL1;Extra spindle poles-like 1 protein;Separase;ESPL1;ESP1, KIAA0165; ESPL1 ESP1, SEPA extra spindle pole bodies like 1, separase separin|caspase-like protein ESPL1|extra spindle pole bodies 1, separase|extra spindle pole bodies homolog 1|extra spindle poles like 1|extra spindle poles-like 1 protein|separin, cysteine protease
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ESPL1, check out the ESPL1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ESPL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Separase (A795) ESPL1 Antibody (A04364-1)
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