Click image to see more details
Product Info Summary
| SKU: | A06093-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-LMO1 Antibody Picoband®
SKU/Catalog Number
A06093-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-LMO1 Antibody Picoband® catalog # A06093-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LMO1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06093-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human LMO1, different from the related mouse sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A06093-1 is reactive to LMO1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
18 kDa
Calculated molecular weight
17828 MW
Background of LMO1
Rhombotin-1 is a protein that in humans is encoded by the LMO1 gene. This locus encodes a transcriptional regulator that contains two cysteine-rich LIM domains but lacks a DNA-binding domain. LIM domains may play a role in protein interactions; thus the encoded protein may regulate transcription by competitively binding to specific DNA-binding transcription factors. Alterations at this locus have been associated with acute lymphoblastic T-cell leukemia. Chromosomal rearrangements have been observed between this locus and at least two loci, the delta subunit of the T-cell antigen receptor gene and the LIM domain binding 1 gene. Alternatively spliced transcript variants have been described.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06093-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: rat brain tissue, NIH3T3 whole cell, HELA whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of LMO1 using anti-LMO1 antibody (A06093-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
lane 1: rat brain tissue lysate,
lane 2: NIH3T3 whole cell lysates,
lane 3: HELA whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LMO1 antigen affinity purified polyclonal antibody (Catalog # A06093-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LMO1 at approximately 18KD. The expected band size for LMO1 is at 18KD.
Protein Target Info & Infographic
Gene/Protein Information For LMO1 (Source: Uniprot.org, NCBI)
Gene Name
LMO1
Full Name
Rhombotin-1
Weight
17828 MW
Alternative Names
Rhombotin-1;Cysteine-rich protein TTG-1;LIM domain only protein 1;LMO-1;T-cell translocation protein 1;LMO1;RBTN1, RHOM1, TTG1; LMO1 RBTN1, RHOM1, TTG1 LIM domain only 1 rhombotin-1|LIM domain only 1 (rhombotin 1)|LIM domain only protein 1|LMO-1|T-cell translocation gene 1|T-cell translocation protein 1|cysteine-rich protein TTG-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LMO1, check out the LMO1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LMO1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LMO1 Antibody Picoband® (A06093-1)
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Customer Q&As
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3 Customer Q&As for Anti-LMO1 Antibody Picoband®
Question
Is a blocking peptide available for product anti-LMO1 antibody (A06093-1)?
Verified Customer
Verified customer
Asked: 2020-03-10
Answer
We do provide the blocking peptide for product anti-LMO1 antibody (A06093-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-03-10
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for eye using anti-LMO1 antibody A06093-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-09
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-09
Question
We are currently using anti-LMO1 antibody A06093-1 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
N. Huang
Verified customer
Asked: 2015-09-18
Answer
The anti-LMO1 antibody (A06093-1) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-09-18
