Anti-SEMA3A (P737) Antibody

Product Name

Anti-SEMA3A (P737) Antibody

View all Semaphorin 3A Antibodies

SKU/Catalog Number

A01761

Size

100ul

Form

Liquid

Description

Boster Bio Anti-SEMA3A (P737) Antibody catalog # A01761. Tested in WB,IHC applications. This antibody reacts with Human,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SEMA3A (P737) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01761)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human MAP1B protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01761 is reactive to SEMA3A in Human, Rat

Applications

A01761 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

88889 MW

Background of Semaphorin 3A

Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.

Lixin Zhu, Am J Physiol Cell Physiol, Jun 2005; 288: C1242 - C1254.
H.J. Spence, Hum. Mol. Genet., Aug 2004; 13: 1657 - 1668.
Vera L¨²cia Bonilha, J. Cell Biol., Dec 1999; 147: 1533.
M Berryman, J. Cell Biol., Dec 1995; 131: 1231.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:1000
IHC: 1:50-1:200

Validation Images & Assay Conditions

Click image to see more details

Figure 2. Immunohistochemistry validation of SEMA3A using Anti-SEMA3A (P737) Antibody (A01761).

Immunohistochemistry (IHC) analyzes of SEMA3A (P737) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
For more protocol information of IHC

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Figure 1. Western blotting validation for Anti-SEMA3A (P737) Antibody A01761

Western blot (WB) analysis of SEMA3A (P737) pAb at 1:1000 dilution
Lane1:HEK293T whole cell lysate(40ug)
Lane2:A549 whole cell lysate(40ug)
Lane3:PC3 whole cell lysate(40ug)
Lane4:C6 whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For SEMA3A (Source: Uniprot.org, NCBI)

Alternative Names

(semaphorin) 3A; coll-1; collapsin 1; Hsema-I; Hsema-III; MGC133243; sema domain, immunoglobulin domain (Ig), short basic domain, secreted; Sema III; SEMA1; Sema3A; SEMAD; SEMAIII; SEMAL; Semaphorin 3A; semaphorin D; Semaphorin III; semaphorin L; semaphorin-3A; SemD SEMA3A COLL1, HH16, Hsema-I, Hsema-III, SEMA1, SEMAD, SEMAIII, SEMAL, SemD, coll-1 semaphorin 3A semaphorin-3A|collapsin 1|sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A|semaphorin D|semaphorin III|semaphorin L

For more info on SEMA3A, check out the SEMA3A Infographic

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In this infographic, you will see the following information for SEMA3A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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